Evaluation of transgenic and non-transgenic soybean plants cultivated in soil treated with silver nanoparticles.

• Grant number: 13/15646-3
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): December 01, 2013
• Effective date (End): September 30, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Marco Aurelio Zezzi Arruda
• Grantee: Rodrigo Moretto Galazzi
• Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 16/18373-6 - Comparative omics through hyphenated techniques focusing on metal complexes in transgenic and non-transgenic soybean after cultivation with silver nanoparticles., BE.EP.DR

Abstract

Due to the increased use of nanoparticles, especially silver nanoparticles (AgNPS) in different areas and with different purposes, the scientific community has warned about the possible adverse effects that these can have on living beings, since they may be present in environment. Thus, some studies involving the cultivation of plants with AgNPS have been undertaken to evaluate the possible changes that occur in these organisms. Soybean [Glycine max (L.) Merrill], a crop of great value, is widely used in the fields of food and biofuels due to its properties. With the high demand for this plant, several species have been developed in order to improve the nutritional quality, productivity and speed of cultivation, as well as increased resistance to pests. Accordingly, we developed the Roundup Ready® (RR) resistant to glyphosate, a widely herbicide employed in the world. However, genetic modification can alter the proteome of soybean seeds, as well as metallome the plant. Thus, this project aims to expand the information at proteomic and metallomic level of transgenic and non-transgenic soybean plants cultivated with AgNPS. For this, separation, quantification and identification techniques among them 2-D DIGE and ICP-MS will be employed.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CAMPOS, BRUNA K.; GALAZZI, RODRIGO M.; DOS SANTOS, BRUNA M.; BALBUENA, TIAGO S.; DOS SANTOS, FABIO N.; MOKOCHINSKI, JOAO B.; EBERLIN, MARCOS N.; ARRUDA, MARCO A. Z. Comparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 202, OCT 1 2020. Web of Science Citations: 0.

GALAZZI, RODRIGO MORETTO; LOPES JUNIOR, CICERO ALVES; DE LIMA, TATIANI BRENELI; GOZZO, FABIO CESAR; ZEZZI ARRUDA, MARCO AURELIO. Evaluation of some effects on plant metabolism through proteins and enzymes in transgenic and non-transgenic soybeans after cultivation with silver nanoparticles. JOURNAL OF PROTEOMICS, v. 191, n. SI, p. 88-106, JAN 16 2019. Web of Science Citations: 2.

GALAZZI, RODRIGO MORETTO; ZEZZI ARRUDA, MARCO AURELIO. Evaluation of changes in the macro and micronutrients homeostasis of transgenic and non-transgenic soybean plants after cultivation with silver nanoparticles through ionomic approaches. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, v. 48, p. 181-187, 2018. Web of Science Citations: 2.

CAPALDI ARRUDA, SANDRA CRISTINA; DINIZ SILVA, ALISSON LUIZ; GALAZZI, RODRIGO MORETTO; AZEVEDO, RICARDO ANTUNES; ZEZZI ARRUDA, MARCO AURELIO. Nanoparticles applied to plant science: A review. Talanta, v. 131, p. 693-705, JAN 2015. Web of Science Citations: 94.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GALAZZI, Rodrigo Moretto. Omic strategies in the evaluation of transgenic and non-transgenic soybean plants after cultivation with silver nanoparticles. 2017. Doctoral Thesis - Universidade Estadual de Campinas, Instituto de Química.