Due to the increased use of nanoparticles, especially silver nanoparticles (AgNPS) in different areas and with different purposes, the scientific community has warned about the possible adverse effects that these can have on living beings, since they may be present in environment. Thus, some studies involving the cultivation of plants with AgNPS have been undertaken to evaluate the possible changes that occur in these organisms. Soybean [Glycine max (L.) Merrill], a crop of great value, is widely used in the fields of food and biofuels due to its properties. With the high demand for this plant, several species have been developed in order to improve the nutritional quality, productivity and speed of cultivation, as well as increased resistance to pests. Accordingly, we developed the Roundup Ready® (RR) resistant to glyphosate, a widely herbicide employed in the world. However, genetic modification can alter the proteome of soybean seeds, as well as metallome the plant. Thus, this project aims to expand the information at proteomic and metallomic level of transgenic and non-transgenic soybean plants cultivated with AgNPS. For this, separation, quantification and identification techniques among them 2-D DIGE and ICP-MS will be employed.
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