|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||November 01, 2013|
|Effective date (End):||February 28, 2015|
|Field of knowledge:||Agronomical Sciences - Agronomy|
|Principal researcher:||Marli de Fátima Fiore|
|Grantee:||Gabriela Machineski da Silva|
|Home Institution:||Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil|
The alkaline-saline lakes (salines) of Pantanal da Nhecolândia, Mato Grosso do Sul state (MS), are considered extreme environments due to their high values of pH and salt concentration. Considering the limited information regarding the composition of the microorganisms diversity of these lakes, this research plan aims to generate information on bacterial diversity in alkaline-salinelakes of the Pantanal da Nhecolândia-MS, Brazil.Water samples were collected in two different lakes, which differ about the presence or absence of algae and cyanobacteria blooms throughout the year. Salina Verde is characterized by constant blooms and in Salina Preta this phenomenon never occurs. Samples were collected at two depths (surface and bottom) and two times during the day. The total DNA of the samples will be extracted and bacterial groups present in this environment and the variation of these communities composition will be accessed using the pirosequencing of 16S rRNA gene. Quantitative PCR analysis of the 16S rRNA and nifH genes will be applied to quantify, respectively, total bacterial and diazotrophic community. Furthermore, we will screen, by PCR, cloning and sequencing, some genes involved in biological cycles of the arsenic, selenium and mercury, which are commonly found in these salines. This study will contribute to the knowledgement of bacterial diversity and understanding of biogeochemical processes in brasilian alkaline-saline lakes.