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Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS)

Grant number: 13/15879-8
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Health Sciences - Collective Health
Principal Investigator:Cinara de Cássia Brandão de Mattos
Grantee:Fernando Henrique Antunes Murata
Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Toxoplasmosis , a disease resulting from infection with Toxoplasma gondii , is clinically manifested in the brain forms , congenital and ocular . The prevalence of this disease varies in different areas around the world and depends on several factors . Epidemiological studies conducted in different Brazilian states in recent decades have revealed wide variability in rates of infection with T. gondii. In recent years our group has shown that rates of infection by T. gondii in pregnant women from the northwest region of São Paulo are approximately 65.0 % , the rate of congenital infection in São José do Rio Preto , SP is 2.3 % and ocular toxoplasmosis is approximately 27 % among patients suffering from eye diseases . The genetic characterization of T. gondii performed in human specimens reveals a great diversity and attempts to clarify the relationship between the different strains and human infection , including our region . The aim of this project is to investigate and characterize the infection by T. gondii in their serologic and molecular aspects in the northwest of São Paulo . Its specific objectives include: 1.Validar commercial serological tests used in the identification of anti -T . gondii in groups of patients with suspected or clinical diagnosis of ocular toxoplasmosis , congenital or gestational 2. To evaluate the applicability of these patients cPCR , Nested PCR and qPCR in biological samples ( peripheral blood and amniotic fluid ), 3. Genotypically characterized strains of T. gondii in the region using the PCR - RFLP method and establish relationship with the clinical features of patients affected ( Partnership abroad) . Will be selected serum samples and genomic DNA of patients with clinical diagnosis of ocular toxoplasmosis , pregnancy and congenital stored in the Laboratory of Immunogenetics , Department of Molecular Biology FAMERP whose epidemiological data of the same patients were collected . The methods commercial ELISA ( DiaSorin ) , ELFA ( Biomerieux ) and HALF (Abbott ) will be used for the identification of IgA , IgM and IgG anti- T. gondii and the identification of the genomic DNA of T. gondii is performed with the use of conventional PCR methods , nested PCR , Real Time PCR and PCR - RFLP . The results of serological and molecular analyzes will be compared . The statistical Ç2 ( with Yates correction ) , Fisher 's exact test and t test ( if applicable ) will be used in comparisons . Also be calculated odds ratio and confidence interval of 95 % , when necessary . The sensitivity, specificity and positive predictive values (PPV ) and negative (NPV ) will also be calculated for each employee molecular and serological testing in the analyzes. The results may contribute to understand the importance of adopting measures for preventing infection by T. gondii , and identify the type of strain that affect patients in the region . They can also guide programs clarification, education, prevention , epidemiology and even treatment of various forms of toxoplasmosis in the northwest of São Paulo .

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE FARIA JUNIOR, GERALDO M.; AYO, CHRISTIANE M.; DE OLIVEIRA, AMANDA P.; LOPES, ALESSANDRO G.; FREDERICO, FABIO B.; SILVEIRA-CARVALHO, APARECIDA P.; PREVIATO, MARIANA; BARBOSA, AMANDA P.; MURATA, FERNANDO H. A.; DE ALMEIDA JUNIOR, GILDASIO CASTELLO; SIQUEIRA, RUBENS CAMARGO; DE MATTOS, LUIZ C.; BRANDAO DE MATTOS, CINARA C. CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis. Acta Tropica, v. 178, p. 276-280, FEB 2018. Web of Science Citations: 1.
CAMILO, LILIAN MUNIZ; PEREIRA-CHIOCCOLA, VERA LUCIA; GAVA, RICARDO; MEIRA-STREJEVITCH, CRISTINA DA SILVA; VIDAL, JOSE ERNESTO; BRANDAO DE MATTOS, CINARA CASSIA; FREDERICO, FABIO BATISTA; DE MATTOS, LUIZ CARLOS; JUNQUEIRA FRANCO SPEGIORIN, LIGIA COSENTINO; ANTUNES MURATA, FERNANDO HENRIQUE; FERREIRA, MARINA NEVES; ULISSES BARBOSA, DEUSENIA MACHADO; GONCALVES, FAUSTO DA SILVA; DIAS, CRISTIANE MORAES; CATELAN, MARCIA WAKAI; SIQUEIRA, RUBENS CAMARGO; PREVIATO, MARIANA; BARBOSA, AMANDA PIRES; CAVALLINI, DANILO; GRP, FAMERP TOXOPLASMA RES. Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in Sao Paulo, Brazil. Brazilian Journal of Infectious Diseases, v. 21, n. 6, p. 638-647, NOV-DEC 2017. Web of Science Citations: 3.
MAIA, M. M.; MEIRA-STREJEVITCH, C. S.; PEREIRA-CHIOCCOLA, V. L.; DE HIPPOLITO, D. D. C.; SILVA, V. O.; BRANDAO DE MATTOS, C. C.; FREDERICO, F. B.; SIQUEIRA, R. C.; DE MATTOS, L. C.; GRP, FAMERP IAL TOXOPLASMA RES. Evaluation of gene expression levels for cytokines in ocular toxoplasmosis. PARASITE IMMUNOLOGY, v. 39, n. 10 OCT 2017. Web of Science Citations: 4.
ANTUNES MURATA, FERNANDO HENRIQUE; FERREIRA, MARINA NEVES; PEREIRA-CHIOCCOLA, VERA LUCIA; JUNQUEIRA FRANCO SPEGIORIN, LIGIA COSENTINO; MEIRA-STREJEVITCH, CRISTINA DA SILVA; GAVA, RICARDO; SILVEIRA-CARVALHO, APARECIDA PERPETUO; DE MATTOS, LUIZ CARLOS; BRANDAO DE MATTOS, CINARA CASSIA; GRP, FAMERP TOXOPLASMA RES. Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in Sao Paulo state, Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 89, n. 1, p. 13-19, SEP 2017. Web of Science Citations: 1.
MEIRA-STREJEVITCH, CRISTINA SILVA; PEREIRA-CHIOCCOLA, VERA LUCIA; MAIA, MARTA MARQUES; CARNIETTO DE HIPPOLITO, DAISE DAMARIS; WANG, HUI-TZU LIN; MOTOIE, GABRIELA; DE SOUZA GOMES, APARECIDA HELENA; KANAMURA, CRISTINA TAKAMI; MARTINES, ROOSECELIS BRASIL; BRANDAO DE MATTOS, CINARA CASSIA; FREDERICO, FABIO BATISTA; DE MATTOS, LUIZ CARLOS; GRP, FAMERP TOXOPLASMA RES. Reference genes for studies in infectious parasitic diseases in five types of human tissues. GENE REPORTS, v. 7, p. 98-105, JUN 2017. Web of Science Citations: 3.
AYO, CHRISTIANE MARIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; BRANDAO DE MATTOS, CINARA DE CASSIA; PREVIATO, MARIANA; BARBOSA, AMANDA PIRES; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; DE MATTOS, LUIZ CARLOS. Ocular toxoplasmosis: susceptibility in respect to the genes encoding the KIR receptors and their HLA class I ligands. SCIENTIFIC REPORTS, v. 6, NOV 9 2016. Web of Science Citations: 4.
ANTUNES MURATA, FERNANDO HENRIQUE; FERREIRA, MARINA NEVES; CAMARGO, NATALIA SAHYOUN; SANTOS, GABRIELA SORIA; JUNQUEIRA FRANCO SPEGIORIN, LIGIA COSENTINO; SILVEIRA-CARVALHO, APARECIDA PERPETUO; PEREIRA-CHIOCCOLA, VERA LUCIA; DE MATTOS, LUIZ CARLOS; BRANDAO DE MATTOS, CINARA CASSIA. Frequency of anti-Toxoplasma gondii IgA, IgM, and IgG antibodies in high-risk pregnancies, in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 49, n. 4, p. 512-514, JUL-AUG 2016. Web of Science Citations: 3.
FERNANDO HENRIQUE ANTUNES MURATA; MARINA NEVES FERREIRA; NATÁLIA SAHYOUN CAMARGO; GABRIELA SORIA SANTOS; LÍGIA COSENTINO JUNQUEIRA FRANCO SPEGIORIN; APARECIDA PERPÉTUO SILVEIRA-CARVALHO; VERA LUCIA PEREIRA-CHIOCCOLA; LUIZ CARLOS DE MATTOS; CINARA CÁSSIA BRANDÃO DE MATTOS. Frequency of anti- Toxoplasma gondii IgA, IgM, and IgG antibodies in high-risk pregnancies, in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 49, n. 4, p. -, Ago. 2016.
AYO, CHRISTIANE MARIA; DA SILVEIRA CAMARGO, ANA VITORIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; PREVIATO, MARIANA; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; BARBOSA, AMANDA PIRES; BRANDAO DE MATTOS, CINARA DE CASSIA; DE MATTOS, LUIZ CARLOS. MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis. PLoS One, v. 10, n. 12 DEC 16 2015. Web of Science Citations: 3.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.