Expression and purification of Cryptosporidium parvum COWP5 recombinant protein aiming the production of polyclonal IgY in chickens for diagnostic purpose

• Grant number: 13/24082-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2014
• End date: August 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcelo Vasconcelos Meireles
• Grantee: Milena Sato de Souza
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated scholarship(s): 15/07147-2 - Cloning and expression of a 41-Kilodalton protein from Cryptosporidium parvum in Escherichia coli, BE.EP.DR

Abstract

Cryptosporidium spp. are protozoa which infect a wide variety of vertebrates. Cryptosporidium parvum has many animal hosts, including humans, and is a major etiological agent of diarrhea in ruminants. The Cryptosporidium oocysts wall proteins (COWP) are a group of proteins located on the surface of the oocyst wall and are involved in its structure and resistance of oocysts in environmental conditions. COWP proteins are potential targets for cloning and heterologous expression in E. coli, for the production of recombinant proteins useful for the development of diagnostic methods like direct immunofluorescence assay (DIA), and also for isolation and purification of oocysts using immunological techniques. The aims of this study are the cloning, expression and purification of C. parvum COWP5 recombinant protein, followed by immunization of laying hens in order to obtain IgY anti-COWP5. After obtaining polyclonal IgY, it will be accomplished the development of DIA for detection of C. parvum oocysts. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, Milena Sato de. Production of Cryptosporidium parvum recombinant proteins aiming the development of diagnostic methods. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba Araçatuba.