Validation of substitute method, laboratory and anthropometric, as indicators of insulin resistance by hyperglycemic clamp studied in adolescents. Brazilian Metabolic Syndrome Study (BRAMS)

• Grant number: 13/21476-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: February 01, 2014
• End date: October 31, 2016
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Bruno Geloneze Neto
• Grantee: Cleliani de Cassia da Silva
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Grounding: In parallel with the worldwide obesity epidemics, there is an alarming increase in the prevalence of insulin resistance (IR) and metabolic syndrome among children and adolescents, which are disease states of great and growing importance in terms of morbidity and mortality. Therefore, it becomes important to identify practical methods, accurate and applicable in population studies and in clinical practice to predict insulin resistance in adolescents, because early detection provides healthcare professionals the opportunity to adopt prophylactic and therapeutic measurements.Objective: Validate substitutive methods, laboratory and anthropometric, as insulin resistance indicators studied by the hyperglycemic clamp in adolescents with different levels of adiposity and pubertal stages.Methods: Multicentre cross-sectional study of 835 adolescents (10 to 19 years). There will be assessed: pubertal stage, blood pressure, waist and neck circumference (NC), sagittal abdominal diameter (SAD), BMI z-score, fasting glucose, fasting insulin, total cholesterol and fractions, uric acid, liver enzymes and lean mass. The IR will be assessed by the Homeostasis Model Assessment-adiponectin (HOMA-AD) and by the hyperglycemic clamp. The correlation between two variables will be evaluated by partial correlation coefficient adjusted for pubertal stage. The association between the SAD, the NC and HOMA-AD with the clamp will be evaluated by chi-square test. The degree of agreement between the SAD, the NC and HOMA-AD with the clamp will be evaluated by Kappa coefficient. To evaluate the performance of the NC, the SAD and HOMA-AD to identify insulin resistance assessed by the clamp ROC curves will be constructed and the areas under the curves and their 95% CI will be calculated. To determine the cutoff of NC, the SAD and HOMA-AD to identify we will consider the 90th percentile of the healthy subgroup. Significance level p<0.05.Perspectives: The results of this study should contribute to providing diagnostic tools of insulin resistance applicable in population studies and in clinical practice.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, CLELIANI DE CASSIA; VASQUES, ANA CAROLINA J.; ZAMBON, MARIANA P.; CAMILO, DANIELLA F.; DE BERNARDI RODRIGUES, ANA MARIA; ANTONIO, MARIA ANGELA R. G. M.; GELONEZE, BRUNO; SYNDROME, BRAZILIAN METABOLIC. Sagittal abdominal diameter resembles waist circumference as a surrogate marker of insulin resistance in adolescents-Brazilian Metabolic Syndrome Study. PEDIATRIC DIABETES, v. 19, n. 5, p. 882-891, AUG 2018. (13/21476-3)

DA SILVA, CLELIANI DE CASSIA; ZAMBON, MARIANA PORTO; JUNQUEIRA VASQUES, ANA CAROLINA; CAMILO, DANIELLA FERNANDES; DE BERNARDI RODRIGUES, ANA MARIA; DE GOES MONTEIRO ANTONIO, MARIA ANGELA REIS; DAMASO, ANA RAIMUNDA; TUFIK, SERGIO; DE MELLO, MARCO TULIO; DA SILVEIRA CAMPOS, RAQUEL MUNHOZ; et al. Homeostatic model assessment of adiponectin (HOMA-Adiponectin) as a surrogate measure of insulin resistance in adolescents: Comparison with the hyperglycaemic clamp and homeostatic model assessment of insulin resistance. PLoS One, v. 14, n. 3, MAR 25 2019. (13/21476-3)

CLELIANI DE CASSIA DA SILVA; MARIANA PORTO ZAMBON; ANA CAROLINA JUNQUEIRA VASQUES; DANIELLA FERNANDES CAMILO; MARIA ÂNGELA REIS DE GÓES MONTEIRO ANTONIO; BRUNO GELONEZE. The threshold value for identifying insulin resistance (HOMA-IR) in an admixed adolescent population: A hyperglycemic clamp validated study. ARCHIVES OF ENDOCRINOLOGY METABOLISM, 2023-01-13. (13/21476-3)

DA SILVA, CLELIANI DE CASSIA; ZAMBON, MARIANA PORTO; VASQUES, ANA CAROLINA JUNQUEIRA; CAMILO, DANIELLA FERNANDES; ANTONIO, MARIA ANGELA REIS DE GOES MONTEIRO; GELONEZE, BRUNO. The threshold value for identifying insulin resistance (HOMA-IR) in an admixed adolescent population: A hyperglycemic clamp validated study. ARCHIVES OF ENDOCRINOLOGY METABOLISM, v. 67, n. 1, p. 7-pg., 2023-01-01. (13/21476-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Cleliani de Cassia da. Anthropometric and laboratory surrogate markers of insulin resistance compared to the hyperglycemic clamp in adolescents: Brazilian Metabolic Syndrome (BRAMS) Study. 2017. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.