Evaluation of the synergistic effect in antinociceptive and anti-inflammatory activities of the dichloromethane extract of Pterodon pubescens Benth. seeds and the essential oil of Cordia verbenacea DC

• Grant number: 13/15709-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2014
• End date: August 31, 2015
• Field of knowledge: Health Sciences - Pharmacy - Pharmacognosy
• Principal Investigator: Mary Ann Foglio
• Grantee: Rosanna Tarkany Basting
• Host Institution: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA). Universidade Estadual de Campinas (UNICAMP). Paulínia , SP, Brazil

Abstract

Inflammation is a complex immune response related to tissue damage and / or cell stimulation caused by chemical, physical, immunological or microbial. In general, tissue damage triggers a local inflammatory reaction recruiting leukocytes, which release inflammatory mediators. These substances are able to sensitize nociceptors. After synaptic transmission and signal modulation by nociceptive sensory neurons, these signals are perceived as "pain." Non Steroidal Anti-inflammatory drugs (NSAIDs) are used to control inflammation, inhibiting the inflammatory mediators, but can cause side effects such as stomach ulcers and cardiovascular damage. The studies of new drugs derived from medicinal plants are the starting point for the development of new drugs and improving the generation of compounds with therapeutic potential. The use of phytocomplexs can be an alternative for the treatment of chronic inflammatory diseases. Cordia verbenacea DC (Cv) and Pterodon pubescens Benth. (Pp) are commonly used in the treatment of various inflammatory diseases. Cordia verbenacea has proven anti-inflammatory oral and topical associated with low toxicity, and antimicrobial, antiulcer and anti-edematogenic. The dichloromethane extract of seeds from Pterodon pubescens Benth. has proven anti-inflammatory and antinociceptive activities, due to the presence of furan diterpenes (vouacapan) and geranylgeraniol compounds, suggesting a synergistic effect between them. Previous in vivo study conducted by our research group demonstrated that oral administration of the mixture of dichloromethane extract of seeds (Pp) and essential oil (Cv) showed greater anti-inflammatory effect compared with the groups treated with extracts separated. In the evaluation of the mixture, in the fourth hour after the induction of paw edema inhibition of edema was around 20% higher. In the evaluation at the sixth hour inhibition of edema was around 15% compared with the groups treated only with the extracts separately. There was also an extension of the pharmacological activity, remaining effective after 72 h, with inhibition of about 70% after 48 and 72 hours of the procedure (*p<0.05, Dunnett's test).Therefore, based on pharmacological data on species and preliminary results observed, the aim of this study is to evaluate the synergistic effect between the crude dichloromethane extract of seeds Pterodon pubescens Benth. and the essential oil of Cordia verbenacea DC front antinociceptive and anti-inflammatory in experimental models.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BASTING, ROSANNA TARKANY; SPINDOLA, HUMBERTO MOREIRA; DE OLIVEIRA SOUSA, ILZA MARIA; ALMEIDA QUEIROZ, NUBIA DE CASSIA; TRIGO, JOSE ROBERTO; DE CARVALHO, JOAO ERNESTO; FOGLIO, MARY ANN. Pterodon pubescens and Cordia verbenacea association promotes a synergistic response in antinociceptive model and improves the anti-inflammatory results in animal models. BIOMEDICINE & PHARMACOTHERAPY, v. 112, APR 2019. (15/08600-2, 13/15709-5)