"Envolvimento dos glicocorticoides na inflamação pulmonar aguda induzida por lipopolissacarídeo (LPS) em ratos desnutridos durante o período gestacional, em diferentes idades"

• Grant number: 14/01266-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2014
• End date: February 28, 2015
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Maristella de Almeida Vitta Landgraf
• Grantee: Marcelly Mendes Ballico
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 12/51104-8 - Mechanisms involved in reduced acute and allergic lung inflammation in rats exposed to intrauterine undernourishment., AP.JP

Abstract

Intrauterine nourished and undernourished rats, at 8 and 12 weeks old, will receive intranasal instillation of LPS to induce acute lung injury. To characterize the acute lung injury induced by LPS, the inflammatory cell infiltrate present in the bronchoalveolar lavage (BAL) and in peribronchial regions, as well cytokines and lipid mediators production, in lung tissue, will be evaluated; to evaluate the role of glucocorticoids in this inflammatory response model, plasma levels of this hormone will be measured. Furthermore, we will also assess the gene and protein expression of glucocorticoid receptor in the hypothalamus, hippocampus and lung tissue.