Mode of action of proteins Cry1, Cry2 and Vip3 Bacillus thuringiensis in Spodoptera frugiperda and Anticarsia gemmatalis

• Grant number: 13/15128-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2014
• End date: February 20, 2017
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Janete Apparecida Desidério
• Grantee: Camila Soares Figueiredo
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The bacterium Bacillus thuringiensis has great prominence in agriculture, since this behaves as an efficient agent to control insect pests. Due to the different expression proteins with insecticidal activity as Cry proteins, Cyt, chitinase and and more recently proteins second generation know as Vip. The combination of cry and vip genes being expressed simultaneously by means of genetic engineering can enhance the insecticidal activity, delay or prevent the emergence of target pest populations resistant to the action of proteins when used alone. Thus, this work aims to study the toxicity and mode of action of Cry1A proteins, and Cry2Aa Cry2Ab strain T01326 and Vip3Aa50 isolate I131 against neonate larvae of S. frugiperda and A. gemmatalis. It is expected, from the results obtained from the various analyzes of the mode of action of these proteins, which indicate the best combinations that could be used in pyramiding of transgenic plants for the control of these insect pests as well as the handling of the possible emergence of resistance these insects.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FIGUEIREDO, CAMILA SOARES; ZARA, FERNANDO JOSE; DESIDERIO, JANETE APPARECIDA. Effect of the Cry1, Cry2 and Vip3 protein combinations on the control ofAnticarsia gemmatalis(Erebidae) andChrysodeixis includens(Noctuidae) Lepidoptera. INTERNATIONAL JOURNAL OF PEST MANAGEMENT, v. 68, n. 2, p. 9-pg., 2020-09-17. (13/15128-2)

FIGUEIREDO, CAMILA SOARES; NUNES LEMES, ANA RITA; SEBASTIAO, ISIS; DESIDERIO, JANETE APPARECIDA. Synergism of the Bacillus thuringiensis Cry1, Cry2, and Vip3 Proteins in Spodoptera frugiperda Control. Applied Biochemistry and Biotechnology, v. 188, n. 3, p. 798-809, JUL 2019. (13/15128-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FIGUEIREDO, Camila Soares. Interaction of proteins CRY1, CRY2 and VIP3 Bacillus thuringiensis in control of Anticarsia gemmatalis, Chrysodeixis includens and Spodoptera frugiperda. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.