Preparation of advanced hybrid nanomaterials based on functionalized polypyrrole/graphene/Sb nanoparticles for the laccase-mediated bioelectrochemical detection of endocrine disrupting compounds

• Grant number: 14/02457-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): June 01, 2014
• Effective date (End): May 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Sergio Antonio Spinola Machado
• Grantee: Fernando Henrique Cincotto
• Supervisor: José M. Pingarrón
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Research place: Universidad Complutense de Madrid (UCM), Spain
• Associated to the scholarship: 12/18339-1 - Detection of endocrine disruptors testosterone and estradiol in environmental samples utilizing modified electrodes with graphene, the gold nanoparticles and quantum dots., BP.DR

Abstract

This project proposes to development of laccase-based biosensing platforms for the electrochemical detection of two important endocrine disrupting compounds, 17beta-estradiol and estriol, in environmental samples by using novel hybrid nanomaterials of functionalized polypyrrole/graphene/Sb nanoparticles as transducers. The developed electrode will be characterized by measurements of scanning electron microscopy, X-ray energy dispersive spectroscopy, atomic force microscopy and electrochemical techniques (cyclic voltammetry and electrochemical impedance spectroscopy). The electrochemical behaviour of endocrine disruptors will be studied in different support electrolytes and pH on the surface electrode developed. The sensor optimized will allow increase sensitivity, and consequently the determination of endocrine disruptors estradiol and estriol with low detection limits. The detection of these hormones will be via electrochemical oxidation direct on the sensor surface, in order to develop a sensor precise, sensitive electrocatalytic system with potential of miniaturization and possibility of application "in situ" and real time for the determination of two endocrine disruptors in environmental matrices. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CINCOTTO, FERNANDO H.; CANEVARI, THIAGO C.; MACHADO, SERGIO A. S.; SANCHEZ, ALFREDO; RUIZ BARRIO, MARIA ASUNCION; VILLALONGA, REYNALDO; PINGARRON, JOSE M.. Reduced graphene oxide-Sb2O5 hybrid nanomaterial for the design of a laccase-based amperometric biosensor for estriol. Electrochimica Acta, v. 174, p. 332-339, AUG 20 2015. (14/02457-0, 11/23047-7)