Gastric cancer is the fourth most prevalent and the second cause of cancer death worldwide. Nowadays, Brazil is in the fourth most prevalent in gastric cancer in Latin America. Alterations in methylation patterns or chromatin remodeling have been involved in carcinogenesis due to their crucial role in regulation mechanisms. To identify genes controlled by DNA and/or histone methylation, genes were selected by microarray analysis to be evaluated in 50 pairs of gastric tumor and adjacent non-tumor samples by qRT-PCR. Genes with differential expression will be analyzed in this project for the pattern of methylation in promoter regions by sequencing. These genes will be also evaluated for the pattern of histone H3K9 methylation by chromatin immunoprecipitation. The pattern of DNA and histone methylation will be associated to sex, age, tumor location, H. pylori, tumor extension, limph node metastasis, distant metastasis, and histopathologic type of gastric adenocarcinoma. The current study will originate new and better biological information, as biomarkers, which will enable a better understanding of etiology and physiopathology of this neoplasia with potential medical implication.
News published in Agência FAPESP Newsletter about the scholarship: