Comparison of Possible Alterations in protein Soybean Seeds and Plants Transgenic and Non-Transgenic Coming from Distinct Generations

• Grant number: 13/17514-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date until: April 01, 2014
• End date until: January 31, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Marco Aurelio Zezzi Arruda
• Grantee: Bruna Kauely de Campos
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 09/54676-0 - Oxidative stress induced by metals: new approaches, AP.TEM
• Associated scholarship(s): 15/24668-6 - Quantification of differential proteins between transgenic and non-transgenic soybean seeds using tandem mass spectrometry (label free) approach, BE.EP.DR

Abstract

This research project aims to assess, by comparison, is a genetic alteration caused by transgenesis offers some kind of change in the proteome of soybean seeds with some degree of relatedness. This will be grown and analyzed transgenic soybean seeds and non-transgenic (TS1 and NTS1). New seeds coming plants TS1 and NTS1 will be cultivated and analyzed (TS2 and NTS2), and thus to its fourth generation (TS4 and NTS4). The seeds of TS and NTS will be cultivated under identical conditions as the physico-chemical properties. The object of study for the development of this project will be the proteomics, in which stands a better understand of the proteome, in other words, the map of soybean protein. It is intended to focus the study on the identification of proteins by mass spectrometry (MALDI-QTOF) after a previous stage of the separation by two-dimensional differential gel electrophoresis (2D-DIGE).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CAMPOS, BRUNA K.; GALAZZI, RODRIGO M.; DOS SANTOS, BRUNA M.; BALBUENA, TIAGO S.; DOS SANTOS, FABIO N.; MOKOCHINSKI, JOAO B.; EBERLIN, MARCOS N.; ARRUDA, MARCO A. Z.. Comparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 202, OCT 1 2020. (13/17514-7, 18/25207-0, 16/07384-7, 13/15646-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAMPOS, Bruna Kauely de. Comparative study between generations of transgenic and non-transgenic soybeans seeds using a proteomic and metabolomic approach. 2017. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.