Development and validation of Multiplexed bovine plasma cytokine measurement using multiple reaction monitoring (MRM) mass spectrometry

• Grant number: 14/05405-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 01, 2014
• End date: June 09, 2015
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Flávio Vieira Meirelles
• Grantee: Pedro Ratto Lisboa Pires
• Supervisor: Gene Louise Wijffels
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Institution abroad: CSIRO Animal, Food and Health Sciences, Australia
• Associated to the scholarship: 12/01394-0 - Lipids profile changes associated to pluripotency regulatory mechanisms during mesenchymal cells reprogramming to human induced pluripotent stem cells (IPS), BP.DR

Abstract

Inflammation is a ubiquitous biological process and is often the root cause or a consequence of many acute and chronic diseases and syndromes. Inflammatory processes invoke the involvement of many pro- or anti-inflammatory cytokines. Measurement of cytokines has become one of the primary means of detecting and monitoring inflammatory status and recovery from disease. However, due to their very low levels in blood (pg/mL), enzyme-linked immunosorbant assay (ELISA) approaches are the most common techniques employed to determine cytokine concentrations. More recent developments such as the Luminex assay system allow higher throughput and the ability to measure more than one cytokine at a time in a single sample. However, this approach is still antibody-based and thus dependent on very high quality and well-characterized antibody reagents. The proposed project aims to modernize the quantification of plasma cytokines by application of mass spectrometry using multiple reaction monitoring (MRM) approaches. The use of MRM MS technique is antibody-independent and theoretically can be applied to any protein or peptide in a complex mixture such as plasma. The challenge, as always, for low abundance proteins in biological material, is sample processing and instrument sensitivity to ensure detection and measure of the target. The benefit of this approach is the number of simultaneously detectable proteins without antibodies and others expensive reagents, maintaining high sensitivity and selectivity in high resolution. Another major advantage of this approach is the possibility to be applied to any biological material, occupying a large proportion and value between biotecnias currently used. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDOZA-PORRAS, OMAR; PIRES, PEDRO R. L.; GOSWAMI, HARESHWAR; MEIRELLES, FLAVIO V.; COLGRAVE, MICHELLE L.; WIJFFELS, GENE. Cytokines in the grass, a lesson learnt: Measuring cytokines in plasma using multiple reaction monitoring mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v. 34, n. 9, MAY 15 2020. (14/05405-1)