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Interactions between cariogenic bacteria: susceptibility to chlorhexidine and Confocal Scanning Laser Microscopy using drip-flow biofilm reactor

Grant number: 14/02397-8
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): July 04, 2014
Effective date (End): July 03, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Fernanda Lourenção Brighenti
Grantee:Rosa Virginia Dutra de Oliveira
Supervisor abroad: Garth A. James
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Local de pesquisa : Montana State University (MSU), United States  
Associated to the scholarship:12/17236-4 - Microbial interactions between cariogenic bacteria: evaluation of growth, metabolic activity, biochemical composition and susceptibility to chlorhexidine of biofilms formed in vitro, BP.DR

Abstract

The ideal methodology to test the biofilms susceptibility to antimicrobial agents is using a model that simulates oral environment. An effective approach is the use of reactors, in which biofilms under the influence of a constant flow of culture medium. This study aims to evaluate the interaction between Streptococcus mutans ATCC 35688 with two other cariogenic microorganisms: Actinomyces naeslundii ATCC 19039 and Streptococcus sobrinus ATCC 33478, in biofilms formed in vitro. 10 mL of bacterial suspension containing 107 CFU/mL will be inoculated on the top of glass slides (n=6) placed on drip-flow biofilm reactor. A flow rate of 5 ml/h of 10%-strength BHI will be used and biofilms will be incubated at 37°C for 48h. Biofilms will be treated with 0.5 mL of 0.12% chlorhexidine. Immediate effect of chlorhexidine and regrowth after 24 h will be evaluated by viable counts in specific medium. Another group of biofilms will be cultivated under the same conditions for analysis by confocal laser scanning microscopy. Data obtained will be statistically analyzed using COMSTAT, considering the significance level of 5%. (AU)