Interactions between cariogenic bacteria: susceptibility to chlorhexidine and Confocal Scanning Laser Microscopy using drip-flow biofilm reactor

• Grant number: 14/02397-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): July 04, 2014
• Effective date (End): July 03, 2015
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Fernanda Lourenção Brighenti
• Grantee: Rosa Virginia Dutra de Oliveira
• Supervisor: Garth A. James
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Research place: Montana State University (MSU), United States
• Associated to the scholarship: 12/17236-4 - Microbial interactions between cariogenic bacteria: evaluation of growth, metabolic activity, biochemical composition and susceptibility to chlorhexidine of biofilms formed in vitro, BP.DR

Abstract

The ideal methodology to test the biofilms susceptibility to antimicrobial agents is using a model that simulates oral environment. An effective approach is the use of reactors, in which biofilms under the influence of a constant flow of culture medium. This study aims to evaluate the interaction between Streptococcus mutans ATCC 35688 with two other cariogenic microorganisms: Actinomyces naeslundii ATCC 19039 and Streptococcus sobrinus ATCC 33478, in biofilms formed in vitro. 10 mL of bacterial suspension containing 107 CFU/mL will be inoculated on the top of glass slides (n=6) placed on drip-flow biofilm reactor. A flow rate of 5 ml/h of 10%-strength BHI will be used and biofilms will be incubated at 37°C for 48h. Biofilms will be treated with 0.5 mL of 0.12% chlorhexidine. Immediate effect of chlorhexidine and regrowth after 24 h will be evaluated by viable counts in specific medium. Another group of biofilms will be cultivated under the same conditions for analysis by confocal laser scanning microscopy. Data obtained will be statistically analyzed using COMSTAT, considering the significance level of 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE FARIAS, ALINE LEITE; DE CARVALHO, LORENA PETRUCELLI FREIRE; MENDEZ, DANIELA ALEJANDRA CUSICANQUI; CRUVINEL, THIAGO; BRIGHENTI, FERNANDA LOURENCAO. Characterization of polymicrobial biofilms obtained from saliva or carious lesions in dentin. BIOFOULING, v. 36, n. 7, p. 11-pg., 2020-10-01. (14/02397-8, 13/12326-8, 17/25904-0, 12/17236-4)

DUTRA DE OLIVEIRA, ROSA VIRGINIA; SAMPAIO BONAFE, FERNANDA SALLOUME; PALOMARI SPOLIDORIO, DENISE MADALENA; KOGA-ITO, CRISTIANE YUMI; DE FARIAS, ALINE LEITE; KIRKER, KELLY R.; JAMES, GARTH A.; BRIGHENTI, FERNANDA LOURENCAO. Streptococcus mutans and Actinomyces naeslundii Interaction in Dual-Species Biofilm. MICROORGANISMS, v. 8, n. 2, FEB 2020. (12/17236-4, 14/02397-8, 13/12326-8)