Evolutionary patterns of sex chromosome systems in crickets: an integrated analysis using cytogenetics and genomics

• Grant number: 14/02038-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2014
• End date: December 31, 2017
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Diogo Cavalcanti Cabral de Mello
• Grantee: Octavio Manuel Palacios Gimenez
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated scholarship(s): 16/01506-3 - Uncovering the sex-biased genes expression in Orthoptera, using as model the cricket Gryllus assimilis, BE.EP.DR

Abstract

The analysis of experimental models for knowledge of sex chromosomes evolution progressively advance and currently the molecular cytogenetic and genomic data has been explored for this proposes. In crickets, although few species have been studied at chromosomal point to view the group presents a wide diversity of sex determination chromosomal systems, occurring X0/XX, neo-XY/XX, neo-X1X2Y/X1X1X2X2 and X1X20/X1X1X2X2, originated by distinct chromosomal rearrangements between autosomes and sex chromosomes. Due the occurrence of distinct mechanisms, in distinct stage of differentiation, the crickets could be good experimental model to the understanding of origin/diversification of sex chromosomes. Thus, the objective of this work is to analyze the structure and evolutionary patterns of distinct sex chromosome systems based in the analysis of distinct repetitive DNAs, such multigene families, satellite DNAs, microsatellites, transposable elements, microdissection and chromosome painting, besides the use of genomic data obtained by sequencing. The model species are Gryllus assimilis (X0), Cicloptyloides americanus (X1X20), Neometrypus badius (neo-XY) and Eneoptera surinamensis (neo-X1X2Y), being E. surinamensis the main biological model. The possibility of the integration of genomic (such as, for example, whole sequenced genomes and isolation of sequences specifically from sex chromosomes) and chromosomal data (obtained from classical cytogenetics and physical chromosome mapping) became real with the advent of new technologies. The possibility of association of these data in crickets, mainly in E. surinamensis will permit a deeper knowledge related to the structure, molecular composition and evolution of sex systems in Orthoptera. These data will permit the proposition of new hypothesis that raise the origin/evolution of derived sex chromosomes and the test of them.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PALACIOS-GIMENEZ, OCTAVIO MANUEL; DIAS, GUILHERME BORGES; DE LIMA, LEONARDO GOMES; KUHN, GUSTAVO CAMPOS E SILVA; RAMOS, ERICA; MARTINS, CESAR; CABRAL-DE-MELLO, DIOGO CAVALCANTI. High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis. SCIENTIFIC REPORTS, v. 7, JUL 25 2017. (14/02038-8, 14/11763-8)

MENEZES-DE-CARVALHO, NAHANNA ZIMMERMANN; PALACIOS-GIMENEZ, OCTAVIO MANUEL; MILANI, DIOGO; CABRAL-DE-MELLO, DIOGO CAVALCANTI. High similarity of U2 snDNA sequence between A and B chromosomes in the grasshopper Abracris flavolineata. Molecular Genetics and Genomics, v. 290, n. 5, p. 1787-1792, OCT 2015. (14/02038-8, 12/01421-7, 11/19481-3)

PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.. Repetitive DNA chromosomal organization in the cricket Cycloptiloides americanus: a case of the unusual X(1)X(2)0 sex chromosome system in Orthoptera. Molecular Genetics and Genomics, v. 290, n. 2, p. 623-631, APR 2015. (14/02038-8, 14/11763-8)

PALACIOS-GIMENEZ, OCTAVIO M.; MILANI, DIOGO; LEMOS, BERNARDO; CASTILLO, ELIO R.; MARTI, DARDO A.; RAMOS, ERICA; MARTINS, CESAR; CABRAL-DE-MELLO, DIOGO C.. Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis. BMC Evolutionary Biology, v. 18, JAN 8 2018. (14/02038-8, 14/11763-8, 15/05246-3)

PALACIOS-GIMENEZ, OCTAVIO M.; ROBERTO CARVALHO, CARLOS; FERRARI SOARES, FERNANDA APARECIDA; CABRAL-DE-MELLO, DIOGO C.. Contrasting the Chromosomal Organization of Repetitive DNAs in Two Gryllidae Crickets with Highly Divergent Karyotypes. PLoS One, v. 10, n. 12, DEC 2 2015. (14/02038-8, 14/11763-8)

BUENO, DANILO; PALACIOS-GIMENEZ, OCTAVIO MANUEL; ANDREA MARTI, DARDO; MARIGUELA, TATIANE CASAGRANDE; CABRAL-DE-MELLO, DIOGO CAVALCANTI. The 5S rDNA in two Abracris grasshoppers (Ommatolampidinae: Acrididae): molecular and chromosomal organization. Molecular Genetics and Genomics, v. 291, n. 4, p. 1607-1613, AUG 2016. (14/11763-8, 14/02038-8, 11/19481-3, 11/18028-3)

CASTILLO, ELIO R. D.; TAFFAREL, ALBERTO; MARONNA, MAXIMILIANO M.; MARTA CIGLIANO, MARIA; PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.; MARTI, DARDO A.. Phylogeny and chromosomal diversification in the Dichroplus elongatus species group (Orthoptera, Melanoplinae). PLoS One, v. 12, n. 2, FEB 28 2017. (14/02038-8, 14/11763-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GIMENEZ, Octavio Manuel Palacios. Patterns of evolution of sex chromosome systems in crickets: an integrated approach between cytogenetics and genomics. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.