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Interaction of Sir2 protein with translation initiation factors in Trypanosoma

Grant number: 14/03714-7
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): May 01, 2014
Effective date (End): March 31, 2016
Field of knowledge:Biological Sciences - Biochemistry - Biochemistry of Microorganisms
Principal researcher:Sergio Schenkman
Grantee:Vinícius Santana Nunes
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated research grant:11/51973-3 - Cell signaling mechanism of Trypanosoma in response to nutritional alterations and genotoxic agents, AP.TEM

Abstract

There are evidences that sirtuins, which correspond to enzymes that remove acetyl groups and modulate several translation initiation factors, modulate the activity of translation initiation factor in eukaryotes. In our laboratory we obtained preliminary evidences that these interactions occur in Trypanosoma cruzi by co-immunoprecipitation assays. We aim to further investigate these interactions. To do this we plan:1- To demonstrate b in vitro assays the the interaction of sir2 of T. cruzi with iF2± and eIF5A and if these interactions depend on their post-translational modifications.2- To study whether the interaction of Sir2 modulates the phosphorylation of T. cruzi eiF2± in parasite extracts.3- To study if Sir2 activity and cellular distribution varies upon different stresses.4- To detect whether eiF5A and/or eiF2a are acetylated under normal or stress conditions.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS MOURA, LEILA; SANTANA NUNES, VINICIUS; GOMES, ANTONIEL A. S.; SOUSA, ANA CAROLINE DE CASTRO NASCIMENTO; FONTES, MARCOS R. M.; SCHENKMAN, SERGIO; MORETTI, NILMAR SILVIO. Mitochondrial Sirtuin TcSir2rp3 Affects TcSODA Activity and Oxidative Stress Response in Trypanosoma cruzi. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, NOV 11 2021. Web of Science Citations: 0.
NUNES, VINICIUS SANTANA; MORETTI, NILMAR SILVIO; DA SILVA, MARCELO SANTOS; ELIAS, MARIA CAROLINA; JANZEN, CHRISTIAN J.; SCHENKMAN, SERGIO. Trimethylation of histone H3K76 by Dot1B enhances cell cycle progression after mitosis in Trypanosoma cruzi. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1867, n. 7 JUL 2020. Web of Science Citations: 0.
LEANDRO DE JESUS, TERESA CRISTINA; NUNES, VINICIUS SANTANA; LOPES, MARIANA DE CAMARGO; MARTIL, DAIANA EVELIN; IWAI, LEO KEI; MORETTI, NILMAR SILVIO; MACHADO, FABRICIO CASTRO; DE LIMA-STEIN, MARIANA L.; THIEMANN, OTAVIO HENRIQUE; ELIAS, MARIA CAROLINA; JANZEN, CHRISTIAN; SCHENKMAN, SERGIO; CHAGAS DA CUNHA, JULIA PINHEIRO. Chromatin Proteomics Reveals Variable Histone Modifications during the Life Cycle of Trypanosoma cruzi. JOURNAL OF PROTEOME RESEARCH, v. 15, n. 6, p. 2039-2051, JUN 2016. Web of Science Citations: 12.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.