The pluripotency is the capacity that some cells posses to ability to generate cells from the three germ layers (ectoderm, mesoderm and endoderm) as well as the germ cells. The embryonic stem cells (ESC) are the most known pluripotent cells, they present a high capacity of cell differentiation and self-renewal. These properties make ESC as potential tools for regenerative medicine, but its use in pratical clinic is not viable, because, in addition to the ethics issues, it is necessary a imunocompatibilite between these cells and the host. Aiming to solve these problems, it was generated the induced pluipotent stem cells (iPSC). These cells, on other hand, are obtained thought nuclear reprogramming by inserting some pluripotency-related factor, initially been these factor the ones known as the four Yamanaka Factors (Oct4, Sox2, c-Myc and Klf4). It was recently discoverd some regulatory RNAs, the microRNAs (miRNAs) that plays an important role on pluripotency maintaining, as well as on cell differentiation process. These miRNAs are small molecules of RNA that act as pós-transcriptional regulators of gene expression by binding to target mRNA resulting in translation inhibition. In addition to pluripotency and cell differentiation process, these miRNAs may regulate many cellular processes like proliferation and cell death among others. It is known that the use of synthetic miRNAs molecules can generate iPSC or increase the efficiency in nuclear reprogramming, as well as to induce stem cells differentiation. The embryonal carcinoma cells (ECC) have similarities to ESC, like pluripotency, in vitro cell differentiation capacity and potential for generate teratomas in vivo. Comparative analysis between the profile of mRNA and miRNA expression in ESC and ECC demonstrated these cells share similarities, thus the use of ECC is widely applied in studying the pluripotent stem cells biology. The present study aims to evaluate the effect of different miRNAs on a human ECC line, NTera-2, by using synthetic precursors (pré-miRs) or inhibitors (anti-miRs) miRNAs molecules, studying the effects of these molecules over cell proliferation, apoptosis and cell differentiation through using different methodologies as qPCR, microarrays and High-Content Analysis/Microscopy (HCS).
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