|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||June 01, 2014|
|Effective date (End):||February 29, 2016|
|Field of knowledge:||Agronomical Sciences - Veterinary Medicine - Animal Pathology|
|Principal Investigator:||Angelo Berchieri Junior|
|Grantee:||Fernanda de Oliveira Barbosa|
|Home Institution:||Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil|
Salmonella spp. is one of the microorganisms of greatest impact on the poultry industry due to economic losses, as well as public health importance. Salmonella Enteritidis does not have a specific host and may cause disease in humans and birds. The ability of SE to colonize the enteric tract and invade their hosts are key factors for stablishing salmonellosis. The flagellum has been thought as an important structure for the pathogenicity of SE. Among its functions are to assist in intestinal colonization and stimulate innate and adaptive immune system. Although there are studies evaluating the role of the flagella in intestinal colonization and systemic invasion by SE, their results are controversial and require further investigation. The present study aims at evaluating the importance of the flagellum and motility of SE for intestinal colonization and systemic invasion in comercial layers. In order to investigate this subject, two SE mutante strains will be constructed. The first mutant will have a deletion in fliC gene (responsible for flagellin, the main component of the flagellar filament). The second mutant, although able to synthesize flagella, will have impaired motility due to inactivation of motB gene. Mutants together with the wild type SE will be inoculated in birds for pathogenesis evaluation.