Nowadays, despite the well-known antioxidant carotenoids, polyphenols and vitamins have already been determined in a wide range of natural sources, their presence in many native or adapted Brazilian fruits still remains unknown. Moreover, the literature about the in vitro scavenging features of the extracts from such typical fruits against reactive oxygen and nitrogen species (ROS and RNS, respectively), as well as the bioavailability of the bioactive compounds involved, is very limited. Hence, this project aims to elucidate new sources of antioxidants, with special attention to carotenoids, polyphenols and vitamins, through the study of fruits from peculiar Brazilian cultivars, such as yellow mombin (Spondias lutea), jelly palm (Butia eriospatha), feijoa (Feijoa sellowiana Berg), black (Rubus spp) and wild (Morus nigra L.) blackberry, grumixama (Eugenia brasiliensis Lam) and orange (Citrus sinensis). The systematic characterization of fruits will be carried out by an untargeted metabolomic approach of carotenoids, polyphenols and vitamins using high performance liquid chromatography analysis coupled to diode array and tandem mass spectrometry detection (HPLC-DAD-MSn), while sugars, organic acids and amino acids would be determined (as their respective trimethylsilyl, TMS, derivatives) by gas chromatography coupled to mass spectrometry (GC-MS) analysis. Moreover, the characteristic in vitro scavenging activities of the considered extracts against specific ROS and RNS will be also studied in detail. The expected contribution from this work is related to the socio-economic development by stimulating the sustainable use of the Brazilian natural resources, such as fruit extracts rich in bioactive compounds, by the food, pharmaceutical and cosmetic industries.
News published in Agência FAPESP Newsletter about the scholarship: