Characterization of a glass ionomer cement containing natural products: in vitro and in vivo studies

• Grant number: 14/01723-9
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): July 01, 2014
• Effective date (End): August 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal researcher: Regina Maria Puppin Rontani
• Grantee: Aline Rogéria Freire de Castilho
• Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated scholarship(s): 15/08871-6 - Antibiofilm effect of a glass ionomer cement containing natural products, BE.EP.PD

Abstract

The goal of this study is to incorporate natural compounds (flavonoids, terpenoid, benzophenone) to glass ionomer cement (GIC) and evaluate their: 1) chemical stability, antibacterial, biological and mechanical properties in vitro; 2) anti-caries activity in rats. To evaluate the material chemical properties Reverse-Phase HPLC will be used to identify and quantify products and subproducts of the natural compounds in fresh and aged GIC. Microbiologic evaluation will consist of an agar diffusion test on cariogenic bacteria for 24 h. The influence of the GICs on biofilms’ inhibition and structural organization will be examined by biochemical assays (biomass, polysaccharide and proteins), laser scanning confocal fluorescence imaging and fluorescence microscopy via the variable pressure scanning electron microscope. Compressive strength, diametral tensile strength, superficial roughness and hardness will be performed in order to analyze mechanical properties. The cytotoxicity of the GICs on oral keratinocyte (Oba-9), oral fibroblast (CRL-7421) and HeLa (CCL-2) will be determined by MTT assay and the influence of GIC in the gene expression. An animal model will be used to evaluate the anti-caries potential. Wistar SPF rats (n=48) will receive sealants of GIC containing the natural compounds, GIC (negative control) or no treatment and then submitted to a cariogenic challenge. Data will be tested for normality and homoscedasticity for selection of the most appropriate statistical test. The significance level will be set at 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, JOZINETE VIEIRA; FREIRES, IRLAN ALMEIDA; CASTILHO, ALINE ROG ERIA; DA CUNHA, MARCOS GUILHERME; ALVES, HARLEY DA SILVA; ROSALEN, PEDRO LUIZ. Antifungal potential of Sideroxylon obtusifolium and Syzygium cumini and their mode of action against Candida albicans. Pharmaceutical Biology, v. 54, n. 10, p. 2312-2319, 2016. (14/01723-9, 13/25080-7, 11/23635-6)

CASTILLO PEDRAZA, MIDIAN C.; ROSALEN, PEDRO L.; FREIRE DE CASTILHO, ALINE ROGERIA; FREIRES, IRIAN DE ALMEIDA; LEITE, LUANA DE SALES; FAUSTOFERRI, ROBERTA C.; QUIVEY, JR., ROBERT G.; KLEIN, I, MARLISE. Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo. JOURNAL OF ORAL MICROBIOLOGY, v. 11, n. 1, JAN 1 2019. (14/01723-9, 17/00753-0, 14/05423-0)

FREIRE DE CASTILHO, ALINE ROGERIA; ROSALEN, PEDRO LUIZ; DE SOUZA ARAUJO, ISAAC JORDAO; KITAGAWA, IGOR LEBEDENCO; GONCALVES DE ARAUJO COSTA, CECILIA ATEM; JANAL, MALVIN N.; ALVES, MARCELO CORREA; DUARTE, SIMONE; LISBOA FILHO, PAULO NORONHA; STIPP, RAFAEL NOBREGA; et al. Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties. PLoS One, v. 14, n. 8, AUG 20 2019. (15/08871-6, 14/01723-9)