Genomic scale metabolic network reconstruction and improvement of polyhydroxyalkanoates production in Pseudomonas sp. (LFM046)

• Grant number: 14/08061-1
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): July 01, 2014
• Effective date (End): June 30, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal researcher: José Gregório Cabrera Gomez
• Grantee: Juliana Cardinali Rezende
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 10/51692-1 - Metabolic engineering applied to biopolymer production, AP.BIOEN.JP
• Associated scholarship(s): 16/24290-6 - Analysis of transcriptional profile from Pseudomonas sp. LFM046 and theirs recombinants producing biopolymer / polyhydroxyalkanoates from glucose based on DNA Microarray and RNA-Seq., BE.EP.PD

Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable polymers with thermoplastic properties produced bymicroorganisms from renewable materials. PHAs containing medium-chain-length monomers (PHAMCL)produced by Pseudomonas sp. have a broad spectrum of use. This proposal aims genomic sequencing andannotation of a new species of Pseudomonas (LFM046) able to produce PHAMCL. Pulsed-fieldelectrophoresis also will be performed for discrimination/comparative analysis from this new species ofPseudomonas in relation to others already known, providing information about the number of chromosomes,plasmids and expected genome size. Furthermore, recombinants strains will be built presenting a controlledexpression of specific genes from the Entner-Doudoroff pathway (ED), in order to evaluate if the expressionlevel of genes from ED interferes on values of PHA yield from carbohydrates. The DNA sequencing usingnext-generation sequencers and genomic annotation of the bacteria will increase our knowledge about thegenotype - phenotype relationship that governs their metabolism. The results generated will be used to buildgenomics scale metabolic network for this strain and theirs recombinants, and to increase the knowledgeabout the metabolic pathways involved in the PHAs production and to guide future projects to thedevelopment of improved strains.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDINALI-REZENDE, JULIANA; DI GENOVA, ALEX; NAHAT, RAFAEL A. T. P. S.; STEINBUECHEL, ALEXANDER; SAGOT, MARIE-FRANCE; COSTA, RAFAEL S.; OLIVEIRA, HENRIQUE C.; TACIRO, MARILDA K.; SILVA, LUIZIANA F.; GOMEZ, JOSE GREGORIO C. The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046. International Journal of Biological Macromolecules, v. 163, p. 240-250, NOV 15 2020. Web of Science Citations: 0.

CARDINALI-REZENDE, JULIANA; RADUAN ALEXANDRINO, PAULO MOISES; PEREIRA DE SOUZA NAHAT, RAFAEL AUGUSTO THEODORO; VIEIRA SANT'ANA, DEBORA PARRINE; SILVA, LUIZIANA FERREIRA; CABRERA GOMEZ, JOSE GREGORIO; TACIRO, MARILDA KEICO. Draft Genome Sequence of Pseudomonas sp. Strain LFM046, a Producer of Medium-Chain-Length Polyhydroxyalkanoate. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 4 JUL-AUG 2015. Web of Science Citations: 0.