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Administration of riluzole, tempol and mesenchymal stem cells in the treatment of ALS in SOD1 G93A mice

Grant number: 13/16168-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2014
Effective date (End): February 02, 2018
Field of knowledge:Biological Sciences - Morphology - Anatomy
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Alexandre Leite Rodrigues de Oliveira
Grantee:Gabriela Bortolança Chiarotto
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):17/02895-6 - Role of MHC Class I on ALS progression in 129Sv-G93A mice, BE.EP.DR

Abstract

The amyotrophic lateral sclerosis is a neurodegenerative disease characterized by selective loss of motor neurons in the spinal cord, brainstem and motor cortex. It is possible that, similar than occurs in other neurodegenerative diseases, the pathological mechanism underlying ALS is a set of cellular and biochemical alterations that ultimately trigger the degeneration of motor neurons. The treatment is mainly based on symptomatic measures and pharmacological therapy with riluzole is not curative. Thus, it is of utmost importance the development of new therapeutic strategies. Alternatively, there is the prospect of using cyclic nitroxides such as Tempol, since they are multifunctional antioxidants that have low toxicity in experimental animals. Another option is a stem cell therapy to replace degenerating neurons, or prevent / delay the process of neuronal death. The objective of this study is to verify if the interaction between riluzole, tempol and mesenchymal stem cells have therapeutic potential in SOD1 G93A transgenic mice. Treatment with riluzole (8mg/kg), Tempol (24mg/kg) and mesenchymal stem cells will start at 70 days of life transgenic animals. Animal control will receive saline as a vehicle. To determine the age of onset of the disease and differences in the progression will be used Rotarod motor test and body weight of the animals. The survival of animals will also be analyzed. After euthanasia, samples will be processed for the realization of the following techniques: Nissl staining, immunohistochemistry, transmission electron microscopy and qRT-PCR. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHIAROTTO, GABRIELA BORTOLANCA; NARDO, GIOVANNI; TROLESE, MARIA CHIARA; FRANCA, JR., MARCONDES CAVALCANTE; BENDOTTI, CATERINA; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. The Emerging Role of the Major Histocompatibility Complex Class I in Amyotrophic Lateral Sclerosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 18, n. 11, . (13/16168-8, 14/06892-3, 17/02895-6)
SPEJO, A. B.; CHIAROTTO, G. B.; FERREIRA, A. D. F.; GOMES, D. A.; FERREIRA JR, R. S.; BARRAVIERA, B.; OLIVEIRA, A. L. R.. Neuroprotection and immunomodulation following intraspinal axotomy of motoneurons by treatment with adult mesenchymal stem cells. JOURNAL OF NEUROINFLAMMATION, v. 15, . (13/16168-8, 14/06892-3, 12/22750-9)
CHIAROTTO, GABRIELA BORTOLANCA; CARTAROZZI, LUCIANA POLITTI; PEREZ, MATHEUS; BISCOLA, NATALIA PERUSSI; SPEJO, ALINE BARROSO; GUBERT, FERNANDA; FRANCA JUNIOR, MARCONDES; MENDEZ-OTERO, ROSALIA; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. Tempol improves neuroinflammation and delays motor dysfunction in a mouse model (SOD1(G93A)) of ALS. JOURNAL OF NEUROINFLAMMATION, v. 16, n. 1, . (13/16168-8, 17/02895-6, 18/05006-0, 14/06892-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CHIAROTTO, Gabriela Bortolança. Administration of riluzole, tempol and mesenchymal stem cells in treatment of ALS in SOD1G93A mice. 2018. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.