| Grant number: | 13/16168-8 |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| Start date: | June 01, 2014 |
| End date: | February 02, 2018 |
| Field of knowledge: | Biological Sciences - Morphology - Anatomy |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Alexandre Leite Rodrigues de Oliveira |
| Grantee: | Gabriela Bortolança Chiarotto |
| Host Institution: | Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Associated scholarship(s): | 17/02895-6 - Role of MHC Class I on ALS progression in 129Sv-G93A mice, BE.EP.DR |
Abstract The amyotrophic lateral sclerosis is a neurodegenerative disease characterized by selective loss of motor neurons in the spinal cord, brainstem and motor cortex. It is possible that, similar than occurs in other neurodegenerative diseases, the pathological mechanism underlying ALS is a set of cellular and biochemical alterations that ultimately trigger the degeneration of motor neurons. The treatment is mainly based on symptomatic measures and pharmacological therapy with riluzole is not curative. Thus, it is of utmost importance the development of new therapeutic strategies. Alternatively, there is the prospect of using cyclic nitroxides such as Tempol, since they are multifunctional antioxidants that have low toxicity in experimental animals. Another option is a stem cell therapy to replace degenerating neurons, or prevent / delay the process of neuronal death. The objective of this study is to verify if the interaction between riluzole, tempol and mesenchymal stem cells have therapeutic potential in SOD1 G93A transgenic mice. Treatment with riluzole (8mg/kg), Tempol (24mg/kg) and mesenchymal stem cells will start at 70 days of life transgenic animals. Animal control will receive saline as a vehicle. To determine the age of onset of the disease and differences in the progression will be used Rotarod motor test and body weight of the animals. The survival of animals will also be analyzed. After euthanasia, samples will be processed for the realization of the following techniques: Nissl staining, immunohistochemistry, transmission electron microscopy and qRT-PCR. (AU) | |
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