Advanced search
Start date
Betweenand

Co-culture of microorganisms isolated from the rhizosphere of Senna spectabilis aiming to produce new bioactive metabolites

Grant number: 14/05935-0
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): June 01, 2014
Effective date (End): April 01, 2019
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal Investigator:Ian Castro-Gamboa
Grantee:Denise Medeiros Selegato
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery, AP.CEPID
Associated scholarship(s):17/06466-2 - Microbe-metabolomic of endophytic and rhizosphere fungi from Senna spectabilis by nuclear magnetic resonance (NMR), BE.EP.DD

Abstract

The search for new sources of natural products have been responsible for the increased use of analytical tools that enable a rapid and efficient analysis of these complex matrixes and is of fundamental importance in the current bioprospecting programs. In this context, the methodologies associated with dereplication methodologies coupled to spectroscopic state of the art technology as well as different in vitro and in vivo bioassays are accelerating the choosing of biologically promising extracts. Our research group "NuBBE" (Nuclei of Bioassays, Biosynthesis and Ecophysiology of Natural Products) has incorporated experimental strategies and chromatographic methods to select, from our bank of extract, lead candidates for further studies. Recently the use of high performance liquid chromatography (HPLC) hyphenated with high resolution mass spectrometry (HRMS) allowed us to analyze plant and endophytic fungi extracts speeding up the selection of biologically active fractions as well as assisting on chemical composition studies of the known bioactive chemotypes and selecting those with novel molecular characteristics for further separation and purification. Innovative spectroscopic techniques using Nuclear Magnetic Resonance (NMR), recently applied on the study of dynamic complex matrixes, are being integrated with HPLC methods mainly due to their robustness, low sample amounts and spectroscopic versatilities. This project aims to understand the molecular relationships present in complex matrices generating a rational approach to study the coexistence between the rhizosphere and microorganisms that inhabit it, making it a rich source of bioactive metabolites. The rhizosphere of Senna spectabilis (Fabaceae) holds a well-known cytotoxic potential, little studied towards their chemical composition and microbial interaction. Additionally, Senna spectabilis's rhizosphere will be rationally explored from which selected microorganism will be grown in co-cultures aiming to validate their cytotoxic potential, as well as to observe unknown microbial interactions. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALAN C. PILON; DENISE M. SELEGATO; RICHARD P. FERNANDES; PAULA C. P. BUENO; DANIELLE R. PINHO; FAUSTO CARNEVALE NETO; RAFAEL T. FREIRE; IAN CASTRO-GAMBOA; VANDERLAN S. BOLZANI; NORBERTO P. LOPES. METABOLÔMICA DE PLANTAS: MÉTODOS E DESAFIOS. Química Nova, v. 43, n. 3, p. 329-354, Mar. 2020.
SELEGATO, DENISE MEDEIROS; FREIRE, RAFAEL TEIXEIRA; PILON, ALAN CESAR; BIASETTO, CAROLINA RABAL; DE OLIVEIRA, HAROLDO CESAR; DE ABREU, LUCAS MAGALHAES; ARAUJO, ANGELA REGINA; BOLZANI, VANDERLAN DA SILVA; CASTRO-GAMBOA, IAN. Improvement of bioactive metabolite production in microbial cultures-A systems approach by OSMAC and deconvolution-based (HNMR)-H-1 quantification. Magnetic Resonance in Chemistry, v. 57, n. 8, p. 458-471, AUG 2019. Web of Science Citations: 0.
FREIRE, RAFAEL TEIXEIRA; BERO, JOANNE; BEAUFAY, CLAIRE; SELEGATO, DENISE MEDEIROS; COQUEIRO, ALINE; CHOI, YOUNG HAE; QUETIN-LECLERCQ, JOELLE. Identification of antiplasmodial triterpenes from Keetia species using NMR-based metabolic profiling. METABOLOMICS, v. 15, n. 3 MAR 2019. Web of Science Citations: 1.
DENISE M. SELEGATO; AFIF F. MONTEIRO; NATÁLIA C. VIEIRA; PATRÍCIA CARDOSO; VICTOR D. PAVANI; VANDERLAN S. BOLZANI; IAN CASTRO-GAMBOA. Update: Biological and Chemical Aspects of Senna spectabilis. Journal of the Brazilian Chemical Society, v. 28, n. 3, p. -, Mar. 2017.
SELEGATO, DENISE M.; MONTEIRO, AFIF F.; VIEIRA, NATALIA C.; CARDOSO, PATRICIA; PAVANI, VICTOR D.; BOLZANI, VANDERLAN S.; CASTRO-GAMBOA, IAN. Update: Biological and Chemical Aspects of Senna spectabilis. Journal of the Brazilian Chemical Society, v. 28, n. 3, SI, p. 415-426, MAR 2017. Web of Science Citations: 1.
DENISE M. SELEGATO; RAFAEL T. FREIRE; ALBERTO TANNÚS; IAN CASTRO-GAMBOA. New Dereplication Method Applied to NMR-Based Metabolomics on Different Fusarium Species Isolated from Rhizosphere of Senna spectabilis. Journal of the Brazilian Chemical Society, v. 27, n. 8, p. -, Ago. 2016.
NETO, FAUSTO CARNEVALE; PILON, ALAN C.; SELEGATO, DENISE M.; FREIRE, RAFAEL T.; GU, HAIWEI; RAFTERY, DANIEL; LOPES, NORBERTO P.; CASTRO-GAMBOA, IAN. Dereplication of Natural Products Using GC-TOF Mass Spectrometry: Improved Metabolite Identification by Spectral Deconvolution Ratio Analysis. FRONTIERS IN MOLECULAR BIOSCIENCES, v. 3, 2016. Web of Science Citations: 5.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SELEGATO, Denise Medeiros. Estudos metabolômicos de fungos associados a plantas : desenvolvimento de métodos para o aumento da produção e identificação de metabólitos secundários bioativos microbianos. 2019. 289 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Química..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.