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Venomics plataform of Pachycondyla villosa proteome, transcriptome, cloning and expression of toxins with biotechnological potential

Grant number: 13/26200-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2014
Effective date (End): January 03, 2018
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Eliane Candiani Arantes Braga
Grantee:Camila Takeno Cologna
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):15/17466-8 - Venomics platform: Pachycondyla villosa venom proteome and integrated analysis of omics data, BE.EP.PD

Abstract

Animal venoms are considered a large natural library, constantly selected and refined by the processes of natural evolution, in which each molecule is endowed with pharmacological property highly valuable for human use. It is estimated that the total number of molecules present in animal venoms is a collection of 40 million different compounds and, despite the efforts made, only 3,000 have been identified and characterized to date. Given these data, the use of animal venoms as a source of biomolecules for the development of new drugs is evident. The concept of turning a toxin to a commercially viable drug is not a new strategy: captopril, developed in the 70s, is the most successful example of a drug derived from animal venom. Despite the commitment, the exploration of these bio-libraries still remains limited. This may be related to the technological limitations that prevent full-scale investigation of these venoms. In addition, the conventional methods used to explore animal venoms are still time-consuming and require large amounts of samples, which restrict the studies for a few species. However, recent improvements in the techniques of molecular biology and mass spectrometry have been contributing to the advance of this promising area. The sensitivity improvement of these instruments allows the study of minimum amounts of sample, but with a wealth of information. This project aims to explore peptides of biotechnological interest present in the venom of the ant Pachycondilyla villosa through the venomics plataform. In this in ascension strategy, a combinatorial approach between transcriptome and proteome form the perfect couple for characterization and description of new toxins of biotechnological interest in a quickly and efficiently way.

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FIGUEIREDO BORDON, KARLA DE CASTRO; COLOGNA, CAMILA TAKENO; FORNARI-BALDO, ELISA CORREA; PINHEIRO-JUNIOR, ERNESTO LOPES; CERNI, FELIPE AUGUSTO; AMORIM, FERNANDA GOBBI; PINO ANJOLETTE, FERNANDO ANTONIO; CORDEIRO, FRANCIELLE ALMEIDA; WIEZEL, GISELE ADRIANO; CARDOSO, IARA AIME; FERREIRA, ISABELA GOBBO; DE OLIVEIRA, ISADORA SOUSA; BOLDRINI-FRANCA, JOHARA; PUCCA, MANUELA BERTO; BALDO, MATEUS AMARAL; ARANTES, ELIANE CANDIANI. From Animal Poisons and Venoms to Medicines: Achievements, Challenges and Perspectives in Drug Discovery. FRONTIERS IN PHARMACOLOGY, v. 11, JUL 24 2020. Web of Science Citations: 0.
AMORIM, FERNANDA GOBBI; LONGHIM, HELOISA TAVONI; COLOGNA, CAMILA TAKENO; DEGUELDRE, MICHEL; DE PAUW, EDWIN; QUINTON, LOIC; ARANTES, ELIANE CANDIANI. Proteome of fraction from Tityus serrulatus venom reveals new enzymes and toxins. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 25, APR 18 2019. Web of Science Citations: 1.
TANAKA SHIBAO, PRISCILA YUMI; COLOGNA, CAMILA TAKENO; MORANDI-FILHO, ROMUALDO; WIEZEL, GISELE ADRIANO; FUJIMURA, PATRICIA TIEMI; UEIRA-VIEIRA, CARLOS; ARANTES, ELIANE CANDIANI. Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, NOV 29 2018. Web of Science Citations: 1.
WIEZEL, GISELE A.; SHIBAO, PRISCILA Y. T.; COLOGNA, CAMILA T.; MORANDI FILHO, ROMUALDO; UEIRA-VIEIRA, CARLOS; DE PAUW, EDWIN; QUINTON, LOIC; ARANTES, ELIANE C. In-Depth Venome of the Brazilian Rattlesnake Crotalus durissus terrificus: An Integrative Approach Combining Its Venom Gland Transcriptome and Venom Proteome. JOURNAL OF PROTEOME RESEARCH, v. 17, n. 11, p. 3941-3958, NOV 2018. Web of Science Citations: 2.
COLOGNA, CAMILA TAKENO; RODRIGUES, RENATA SANTOS; SANTOS, JEAN; DE PAUW, EDWIN; ARANTES, ELIANE CANDIANI; QUINTON, LOIC. Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, p. -, 2018. Web of Science Citations: 4.
BOLDRINI-FRANCA, JOHARA; COLOGNA, CAMILA TAKENO; PUCCA, MANUELA BERTO; FIGUEIREDO BORDON, KARLA DE CASTRO; AMORIM, FERNANDO GOBBI; PINO ANJOLETTE, FERNANDO ANTONIO; CORDEIRO, FRANCIELLE ALMEIDA; WIEZEL, GISELE ADRIANO; CERNI, FELIPE AUGUSTO; PINHEIRO-JUNIOR, ERNESTO LOPES; TANAKA SHIBAO, PRISCILA YUMI; FERREIRA, ISABELA GOBBO; DE OLIVEIRA, ISADORA SOUSA; CARDOSO, LARA AIME; ARANTES, ELIANE CANDIANI. Minor snake venom proteins: Structure, function and potential applications. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1861, n. 4, p. 824-838, APR 2017. Web of Science Citations: 18.
PUCCA, MANUELA B.; PEIGNEUR, STEVE; COLOGNA, CAMILA T.; CERNI, FELIPE A.; ZOCCAL, KARINA F.; BORDON, KARLA DE C. F.; FACCIOLI, LUCIA H.; TYTGAT, JAN; ARANTES, ELIANE C. Electrophysiological characterization of the first Tityus serrulatus alpha-like toxin, Ts5: Evidence of a pro-inflammatory toxin on macrophages. Biochimie, v. 115, p. 8-16, AUG 2015. Web of Science Citations: 12.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.