Use of ruthenium complex cis-[Ru (bpy) 2 (NO2) (NO)] (PF6) 2) to reverse and / or prevent endothelial dysfunction in arterial hypertension

• Grant number: 14/02231-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2014
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Gerson Jhonatan Rodrigues
• Grantee: Izabela Pereira Vatanabe
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 12/24477-8 - Utilization of ruthenium complex as pharmacological strategy to revert and/or prevent the endothelial dysfunction, AP.JP

Abstract

The endothelium is a single layer of cells that lines the interior surface of blood vessels. These cells are responsible for the modulation of vascular tonus. Through the release of nitric oxide (NO), the endothelium shows an important protective role against cardiovascular diseases, producing vasodilation by several mechanisms and generating a number of other effects , whereas at high concentrations may result in toxic effects to the cells. However, the endothelial dysfunction is mainly characterized by decreased ability of endothelial cells to release NO, which may be due to the reaction with superoxide anion (O2-) and formation of peroxynitrite (ONOO-). Nevertheless, in several pathological conditions such as in hypertension, endothelial dysfunction occurs, creating an imbalance between the production/release of vasodilator and vasoconstrictor factors, occurring predominance of contractile factors, leading to increased peripheral vascular resistance and vascular wall hypertrophy. In previous studies, it was found that ruthenium complexes can inactivate O2- and release NO. Thus, this study aims to assess through functional studies and cell culture the drug's potential cis-[Ru(bpy)2(NO2)(NO)](PF6)2 as a pharmacologic strategy to reverse and/or prevent endothelial dysfunction found in the model of hypertension two kidneys clip ( 2R- 1C) and cultured in endothelial cells stimulated with angiotensin II, as well as performing the pharmacological characterization of the dependent effects of O2- removal of and NO release induced by this drug .

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VATANABE, IZABELA PEREIRA; DOS SANTOS RODRIGUES, CARLA NASCIMENTO; BUZINARI, TEREZA CRISTINA; DE MORAES, THIAGO FRANCISCO; DA SILVA, ROBERTO SANTANA; RODRIGUES, GERSON JHONATAN. Ruthenium Complex Improves the Endothelial Function in Aortic Rings From Hypertensive Rats. Arquivos Brasileiros de Cardiologia, v. 109, n. 2, p. 124-131, AUG 2017. (14/02231-2, 12/24477-8)