Structural and functional study of the co-chaperone SGT of Leishmania braziliensis

• Grant number: 14/08786-6
• Support type: Scholarships in Brazil - Master
• Effective date (Start): July 01, 2014
• Effective date (End): June 30, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Julio Cesar Borges
• Grantee: Amanda Lais de Souza Coto
• Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 12/50161-8 - Study of the structure and function of the Hsp90 chaperone with emphasis on its role in cellular homeostasis, AP.TEM

Abstract

Hsp90 are molecular chaperones that are distinguished by acting in diverse cellular processes such as preventing protein aggregation, cell growth and development, stabilization of hormone receptor protein kinase and several other client proteins, the buffering effect against mutations, among others. However, for these proteins efficiently fulfill their duties, they should be modulated by molecular co-chaperones. The SGT is a co-chaperone of Hsp90 as well as Hsp70 that can be divided into three domains: the N-terminal domain, the TPR domain and the C-terminal domain. The TPR domain is responsible for interacting with the Hsp90 as well as Hsp70 C-terminus EEVD motif. The SGT can be found in many organisms, including protozoa of the genus Leishmania spp.. These organisms are responsible for leishmaniasis, a neglected disease that affects thousands of people every year, mainly in developing countries. Studies suggest that in some Leishmania species SGT is essential for growth and viability of the promastigote stage. Therefore, in this project we propose to perform structural and functional studies of the co-chaperone SGT of Leishmania braziliensis with biochemical and biophysical techniques to elucidate their function on Hsp90 molecular cysle. In this way, we aim to enhance the scientific knowledge about this protein poorly studied. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

COTO, Amanda Lais de Souza. Structural and functional studies of the co-chaperone SGT of Leishmania braziliensis. 2016. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Química de São Carlos São Carlos.