Exercício fisico paternal modula a homeostase glicêmica na prole: o papel da metilação do DNA

• Grant number: 14/11369-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: November 01, 2014
• End date: April 30, 2015
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Everardo Magalhães Carneiro
• Grantee: José Maria Costa Júnior
• Supervisor: Klaus H. Kaestner
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: University of Pennsylvania, United States
• Associated to the scholarship: 11/23605-0 - Exercise training in male mice (F0) and epigenetic repercussions on the molecular and functional mechanisms of insulin secretion of the offsprings (F1) fed a high fat diet, BP.DR

Abstract

Paternal exercise modulates offspring glucose homeostasis: The role of DNA methylation.Chronic complications associated with diabetes mellitus result in increasing morbidity, reduce life expectancy, and cause enormous health cost. There is strong evidence that obesity and physical inactivity are the main nongenetic determinants of diabetes. It is also known that alteration in epigenetic mechanisms may be associated with this disease. The effects of maternal nutrition on the metabolic programming of the offspring are well established and, recently, it has been demonstrated an important biological role of the father in this context. Although the beneficial effects of regular exercise are well recognized, little is known regarding exercise performed by the male progenitors in the next generations. Thus, we will analyze the effects of chronic endurance exercise, performed by male progenitors, on: a) glucose homeostasis; b) the molecular and functional mechanisms of insulin secretion in isolated pancreatic islet, c) the insulin sensitivity in skeletal muscle from offspring fed a control or high fat diet and, d) the possible involvement of epigenetic modulation on these processes by either diet or exercise. The last objective will be conducted at the laboratory of Dr. Klaus Kaestner, at the University of Pennsylvania, where we intend to learn the techniques involved in DNA methylation analysis. This technique will be introduced at our laboratory at UNICAMP Keywords: exercise, diabetes mellitus, insulin secretion, pancreatic islets, obesity, epigenetics. (AU)