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The involvement of acetilcoline and glucocorticoids receptors under NFkB transcription in polarization of muscular macrophages after exercise induced damage

Grant number: 14/04504-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2014
Effective date (End): March 21, 2018
Field of knowledge:Health Sciences - Physical Education
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Ronaldo Vagner Thomatieli dos Santos
Grantee:Andre Luis Araujo Minari
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated scholarship(s):16/06817-7 - Interaction between skeletal muscle resident macrophages and myogenic cells after exercise-induced muscle damage in the CX3CR1-GFP/+ mouse, BE.EP.DR


INTRODUCTION: Macrophages (MÕs) and satellite cells (S.C) are closely related to muscular injury and regeneration. After injury (1 to 2 days) MÕs type 1 (M1) provide the clearance of injured cells by phagocytosis and impair myogenesis. During regeneration (4 to 7 days), phagocytosis is concluded and MÕs are simultaneously converted to MÕs type 2 (M2), which restore the process of myogenesis and enable the development of new structures. Besides, few studies shows that acetylcholine and glucorticoides was involve in this polarization under modulation of NFKB, were they inhibited NFKB proinflamatory reaction and promote up-regulation of anti-inflammatory substances .OBJECTIVE: identify the implication nAchR and Glucocorticoids receptors under nuclear factor kappa B (NFkB) pathway in MÕs polarization (M1 to M2) after skeletal muscle injury induced by downhill exercise. METHODS: Appliance of exercise-induced injury protocol by downhill exercise, dissect triceps, extract and isolate subsets of MÕs (M1 and M2) trough flux citometry, account and submit to protein analyses (NFkB proteins, nAchR and Glucocorticoides receptors) by western blot. Besides, we will evaluate the MÕs cytokines production in culture (IL-6, TNF-±, IL-12, IL-10, IL-4, IL-13) by Elisa. All procedures were evaluated 24, 36 and 48 hours after exercise, both in triceps long head (TLH) and triceps Braquii medial head (TB). Animals were distributed in three experimental groups (G24, G36 and G48) and one control group (CTRL). STATISTICAL ANALIZE: The data will be presented in mean and standard deviation and the adequate test will be determinate by normality test Kolmogorov-Smirnov, according experimental designer. (AU)

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