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Genotypic characterization of Toxoplasma gondii in samples of pregnant women in the northwest region of the State of São Paulo

Grant number: 14/01706-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2014
Effective date (End): February 28, 2015
Field of knowledge:Health Sciences - Collective Health
Principal Investigator:Cinara de Cássia Brandão de Mattos
Grantee:Marina Neves Ferreira
Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Toxoplasmosis is a disease resulting from infection by the parasite Toxoplasma gondii that affects much of the world's population . Clinical manifestations in brain , congenital and ocular forms . The prevalence of this disease varies in different areas around the world and depends on climatic factors , eating habits , hygiene and age of affected individuals . In recent years our group has shown that the incidence of infection by T. gondii in pregnant women from the northwest region of the state of São Paulo are approximately 65.0 % , the rate of congenital infection in São José do Rio Preto , SP is 2.3 % . The genetic characterization of T. gondii performed on human samples revealed a great diversity and so we try to clarify the relationship between different strains and human infection , including our region . The aim of this study is to characterize the infection by T. gondii from human biological samples from pregnant women and newborns . Specific objectives include genotypically characterized strains of T. gondii in the region using the method of PCR - RFLP and establish relationship with the clinical picture of affected patients. Serum and genomic DNA of patients with suspected or clinical diagnosis of gestational and congenital toxoplasmosis will be selected , stored at the Laboratory of Immunogenetics , Department of Molecular Biology FAMERP , whose epidemiological data of the same patients were collected . Commercial ELISA methods ( DiaSorin ) and ELFA ( Biomerieux ) will be used for the identification of IgA , IgM and IgG anti -T . gondii. The identification of the genomic DNA of T. gondii is performed with the use of conventional PCR methods , nested PCR , Real Time PCR and PCR- RFLP . The results of serological and molecular analyzes will be compared . Statistical tests Ç2 ( with Yates correction ) , Fisher 's exact test and t test ( if applicable ) will be used in comparisons . You will also calculated the odds ratio and confidence interval of 95 % , when necessary . The results of this project will contribute to the knowledge of the molecular epidemiology of such important forms of toxoplasmosis which is the gestational and congenital , associating them with disease severity . Furthermore, they may contribute to the understanding of congenital infection and outbreak of toxoplasmosis in fetuses and newborns .Keywords : congenital toxoplasmosis , Toxoplasma gondii , epidemiology , diagnosis , genotypes of T. gondii.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMILO, LILIAN MUNIZ; PEREIRA-CHIOCCOLA, VERA LUCIA; GAVA, RICARDO; MEIRA-STREJEVITCH, CRISTINA DA SILVA; VIDAL, JOSE ERNESTO; BRANDAO DE MATTOS, CINARA CASSIA; FREDERICO, FABIO BATISTA; DE MATTOS, LUIZ CARLOS; JUNQUEIRA FRANCO SPEGIORIN, LIGIA COSENTINO; ANTUNES MURATA, FERNANDO HENRIQUE; FERREIRA, MARINA NEVES; ULISSES BARBOSA, DEUSENIA MACHADO; GONCALVES, FAUSTO DA SILVA; DIAS, CRISTIANE MORAES; CATELAN, MARCIA WAKAI; SIQUEIRA, RUBENS CAMARGO; PREVIATO, MARIANA; BARBOSA, AMANDA PIRES; CAVALLINI, DANILO; GRP, FAMERP TOXOPLASMA RES. Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in Sao Paulo, Brazil. Brazilian Journal of Infectious Diseases, v. 21, n. 6, p. 638-647, NOV-DEC 2017. Web of Science Citations: 3.
ANTUNES MURATA, FERNANDO HENRIQUE; FERREIRA, MARINA NEVES; PEREIRA-CHIOCCOLA, VERA LUCIA; JUNQUEIRA FRANCO SPEGIORIN, LIGIA COSENTINO; MEIRA-STREJEVITCH, CRISTINA DA SILVA; GAVA, RICARDO; SILVEIRA-CARVALHO, APARECIDA PERPETUO; DE MATTOS, LUIZ CARLOS; BRANDAO DE MATTOS, CINARA CASSIA; GRP, FAMERP TOXOPLASMA RES. Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in Sao Paulo state, Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 89, n. 1, p. 13-19, SEP 2017. Web of Science Citations: 1.
ANTUNES MURATA, FERNANDO HENRIQUE; FERREIRA, MARINA NEVES; CAMARGO, NATALIA SAHYOUN; SANTOS, GABRIELA SORIA; JUNQUEIRA FRANCO SPEGIORIN, LIGIA COSENTINO; SILVEIRA-CARVALHO, APARECIDA PERPETUO; PEREIRA-CHIOCCOLA, VERA LUCIA; DE MATTOS, LUIZ CARLOS; BRANDAO DE MATTOS, CINARA CASSIA. Frequency of anti-Toxoplasma gondii IgA, IgM, and IgG antibodies in high-risk pregnancies, in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 49, n. 4, p. 512-514, JUL-AUG 2016. Web of Science Citations: 3.
FERNANDO HENRIQUE ANTUNES MURATA; MARINA NEVES FERREIRA; NATÁLIA SAHYOUN CAMARGO; GABRIELA SORIA SANTOS; LÍGIA COSENTINO JUNQUEIRA FRANCO SPEGIORIN; APARECIDA PERPÉTUO SILVEIRA-CARVALHO; VERA LUCIA PEREIRA-CHIOCCOLA; LUIZ CARLOS DE MATTOS; CINARA CÁSSIA BRANDÃO DE MATTOS. Frequency of anti- Toxoplasma gondii IgA, IgM, and IgG antibodies in high-risk pregnancies, in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 49, n. 4, p. -, Ago. 2016.

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