Expression of antiviral factors and endogenous retroviruses constitutive and induced by agonists of toll-like receptors in HIV-1 infected mothers and newborns

• Grant number: 12/21364-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2014
• End date: September 30, 2017
• Field of knowledge: Biological Sciences - Immunology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Notomi Sato
• Grantee: Nátalli Zanete Pereira
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Vertical transmission of HIV occurs in a small percentage of children, with 65-80% of newborns do not become infected even in the absence of antiretroviral therapy. This protection combined with immunological immaturity of newborn makes the study of maternal-fetal interaction a great opportunity to understand the factors that are associated with immune protection of newborns, even during treatment antiretroviral. A project proposal is evaluating the innate immunity of mothers infected with HIV-1 and their newborns, the expression of antiviral factors APOBEC3G and TRIM-5± type I IFN and the immunomodulatory potential of agonists of Toll-like receptors (TLR) in the expression of factors. Also assess the influence of the expression of endogenous retroviruses (HERV-K) in the expression of antiviral factors. To this end, we will analyze the transcriptional expression of APOBEC3G, TRIM-5± and type I IFN and viral factors, HIV Gag and Gag of HERV-K in mononuclear cells from peripheral blood (PBMC) of the mother and the cord and after stimulation with agonists of TLR3, TLR7 and TLR7/8. Parallel, protein expression of APOBEC3G and Gag HIV and HERV-K will be evaluated in PBMCs and after ex vivo activation via TLR7/8. A protein and transcriptional expression of antiviral factors and of HERV-K will also be analyzed in placental tissue. The frequency of CD4 + and CD8 + naive, central memory and peripheral will be assessed for expression of APOBEC3G, IFN-g, TNF-a and MIP-1 an induced by agonist of TLR7 / 8 and Gag HERV-K and HIV-1. The understanding of the innate immune response, with emphasis on antiviral factors and endogenous retrovirus and its modulation by ligands for TLRs, can promote possible correlates of protection and provide grants to develop new formulations of HIV vaccines. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, NATALLI ZANETE; BRANCO, ANNA CLAUDIA CALVIELLI CASTELO; MANFRERE, KELLY CRISTINA GOMES; DE LIMA, JOSENILSON FEITOSA; YOSHIKAWA, FABIO SEITI YAMADA; MILANEZ, HELAINE MARIA BESTETI PIRES MAYER; PEREIRA, NAIURA VIEIRA; SOTTO, MIRIAM NACAGAMI; DUARTE, ALBERTO JOSE DA SILVA; SATO, MARIA NOTOMI. Increased Expression on Innate Immune Factors in Placentas From HIV-Infected Mothers Concurs With Dampened Systemic Immune Activation. FRONTIERS IN IMMUNOLOGY, v. 11, AUG 25 2020. (12/21364-8, 12/18837-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PEREIRA, Nátalli Zanete. Maternal-fetal interaction, antiviral factors and endogenous retroviruses in HIV-1 infection. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.