Structural and biophysical characterization of animal toxins
| Grant number: | 14/11266-4 |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| Effective date (Start): | July 01, 2014 |
| Effective date (End): | June 30, 2015 |
| Field of knowledge: | Biological Sciences - Biophysics |
| Principal Investigator: | Maria Cristina Nonato Costa |
| Grantee: | Ricardo Augusto Pereira de Pádua |
| Home Institution: | Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| Associated research grant: | 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis, AP.TEM |
Abstract Structural biology, a research area focusing the structural characterization of biological macromolecules, has a crucial role in the functional characterization of proteins and nucleic acids. A combination of recombinant DNA, biochemical and biophysical techniques, including specific tools for the determination of 3-dimensional structures provide the basis for the development of studies of the correlation between structure and function. The present project aims the investigation of structure-function relationship of different animal toxins provided and under investigation by our scientific partners, Professor Eliane Candiani Arantes Braga and Professor Suely Vilela, including phospholipases, L-aminoacid oxidases and hialuronidases from different sources. The isolated proteins will be submitted to structural studies by a combination of X-ray diffraction techniques and biophysical techniques such as circular dichroism, thermoflour, and electron paramagnetic resonance. | |