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Effect on metabolites produced by actinomycetes on in vitro digestibility and ruminal fermentation

Grant number: 14/08905-5
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Marcos Veiga dos Santos
Grantee:Bruna Gomes Alves
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Actinomycetes are microorganisms that able to productive active compounds employed as antifungal agents, immunosuppressants, anticarcinogenic, antibacterial, and among them a nigericin, valinomycin and the dynactin have done similar to the ionophore monensin. Our hypothesis is that these active metabolites produced by actinomycetes are similar to monensin for rumen fermentation and improved dry matter digestibility of diets for dairy cows. The aim of this study is to evaluate the effect of nigericin, valinomycin and dynactin on in vitro digestibility of dry matter, pH of rumen fluid, concentration of short chain fatty acids and gas production. For experimental purpose, 100 mL of ruminal liquid by two Holstein cows will be gather. They will fed with control diet of corn silage and concentrate based on soybean meal, urea, corn meal and mineral - vitamin supplement. Doses of metabolities and monensin will be utilized and insert to the rumen fluid for in vitro fermentation in artificial rumen (DAISY®ANKOM-ll). The active metabolites are obtained by micro extraction of isolated actinomycetes growing in eucalyptus and mangrove (Eucal and AMC). From these extracts will be done mass spectrometry to chemical characterization of metabolites. The study will be completely randomized factorial sampling design with 5 treatments (5 x 5), control, monensin, nigericin, valinomycin and dynactin in inclusion levels (0, 2, 4, 6 and 8 mM). The response variables will be: pH, gas, short chain fatty acids and ammonia nitrogen production and in vitro digestibility of dry matter, crude protein and neutral detergent insoluble fiber. The results of in vitro ruminal fermentation will be evaluated as repeated measures by the MIXED of SAS (2001) procedure. Thus, it is expected that this study will develop new alternatives of ionophores monensin compared, which would allow improved ruminal fermentation of dairy cows. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRUNA GOMES ALVES; CRISTIAN MARLON DE MAGALHÃES RODRIGUES MARTINS; ANA PAULA FERRANTI PETI; LUIZ ALBERTO BERALDO DE MORAES; MARCOS VEIGA DOS SANTOS. In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation. REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, v. 48, p. -, 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.