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Correlation of endotoxin levels and symptomatology during endodontic treatment of root canals with primary infections and periapical lesion

Grant number: 14/13636-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2014
Effective date (End): July 31, 2015
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Cláudio Antonio Talge Carvalho
Grantee:Bruna Costa de França Marques
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


Knowing the importance of endotoxin for endodontic treatment, concerning the induction and maintenance of periapical lesions and consequently the success or failure of endodontic treatment, the present study aims to evaluate the levels of endotoxin (LPS) present in root canals with pulp necrosis and radiographically visible periapical lesions during endodontic treatment by mechanical instrumentation (Alternating rotation) and 2.5% sodium hypochlorite. Furthermore, a correlation between the amount of endotoxins with the size of periapical lesions and clinical symptoms will be performed. For this purpose, ten single-root teeth from patients requiring endodontic treatment will be analyzed, which will be instrumented by alternating rotation (Reciproc) and irrigated with sodium hypochlorite 2.5%. Collections of the content of the root canal will be performed immediately after: the coronary opening, the biomechanical preparation, the use of trisodium EDTA 17% and after 14 days of intracanal medication (calcium hydroxide with 2% chlorhexidine gel). For all the collections the endotoxin levels will be measured by the Limulus amoebocyte lysate kinetic chromogenic test. Periapical lesions will be measured by performing CT scans at the beginning of endodontic treatment, for further correlation with the amount of endotoxin and symptoms. Data will be analyzed statistically by Kruskal-Wallis test, Dunn (significance level 5%) and the Pearson linear correlation.

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