The use of Shpol'skii spectroscopy and LC-MS/MS for determination of bisphenol A and analogues in urine samples and exposure assessment in children living in the São Paulo State, Brazil

• Grant number: 13/23710-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2014
• End date: February 28, 2018
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Fernando Barbosa Júnior
• Grantee: Bruno Alves Rocha
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 15/20928-3 - Determination of urinary biomarkers for assessment of Brazilian children exposure to endocrine disrupting chemicals: bisphenols, parabens and phthalates, BE.EP.PD

Abstract

Emerging contaminants refer to chemicals whose interest in studies associated with the occurrence and effects in the environment is recent. In Brazil there is little information related to the exposure to these compounds in the population, which motivated this research project. Among the emerging contaminants stands bisphenol A, which has been widely found in both environmental and biological matrices in several countries. Among the various toxic effects already observed after exposure to this compound those related to the endocrine system may be highlighted.In this sense, this proposal aims to develop methods for the determination of bisphenol A in urine, using a technique already well established in this area, liquid chromatography coupled to mass detector and a technique relatively low cost and simple operation, Shpol'skii spectroscopy. The methods developed will be used for the assessment of exposure to bisphenol A in children in the state of São Paulo, to establish initial/reference values in our population.It is also worth noting that the project will have the collaboration of prof. Dr. Andres Campíglia, University of Central Florida (USA) and prof. Dr. Kannan Kuruntachalan, from the New York State Department of Health (USA), which are performing routine biomonitoring studies with U.S. population. Finally, it is expected that the results of this study can contribute to the knowledge of the levels of exposure to an endocrine disruptor still little studied in Brazil, stimulating public laboratories to implement the methodologies that will be developed in their routine tests.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATHEUS GALLIMBERTI; BRUNO A. ROCHA; VANESSA C. O. SOUZA; ANDRES D. CAMPIGLIA; FERNANDO BARBOSA JR. Determination of Bisphenol A in Paper Products by Synchronous Fluorescence Spectoscopy and Estimation of Daily Exposure. Journal of the Brazilian Chemical Society, v. 31, n. 10, p. 2146-2154, 2020-10-09. (14/00986-6, 12/03465-1, 18/24069-3, 13/23710-3)

ROCHA, BRUNO A.; ASIMAKOPOULOS, ALEXANDROS G.; HONDA, MASATO; DA COSTA, NATTANE L.; BARBOSA, ROMMEL M.; BARBOSA, JR., FERNANDO; KANNAN, KURUNTHACHALAM. Advanced data mining approaches in the assessment of urinary concentrations of bisphenols, chlorophenols, parabens and benzophenones in Brazilian children and their association to DNA damage. Environment International, v. 116, p. 269-277, JUL 2018. (13/23710-3, 15/20928-3)

ROCHA, BRUNO ALVES; BRANDAO DA COSTA, BRUNO RUIZ; PEREZ DE ALBUQUERQUE, NAYARA CRISTINA; MORAES DE OLIVEIRA, ANDERSON RODRIGO; OLIVEIRA SOUZA, JULIANA MARIA; AL-TAMEEMI, MAHA; CAMPIGLIA, ANDRES DOBAL; BARBOSA, JR., FERNANDO. A fast method for bisphenol A and six analogues (S, F, Z, P, AF, AP) determination in urine samples based on dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry. Talanta, v. 154, p. 511-519, JUL 1 2016. (13/23710-3)

GALLIMBERTI, MATHEUS; ROCHA, BRUNO A.; SOUZA, VANESSA C. O.; CAMPIGLIA, ANDRES D.; BARBOSA, FERNANDO, JR.. Determination of Bisphenol A in Paper Products by Synchronous Fluorescence Spectroscopy and Estimation of Daily Exposure. Journal of the Brazilian Chemical Society, v. 31, n. 10, p. 9-pg., 2020-10-01. (12/03465-1, 14/00986-6, 18/24069-3, 13/23710-3)

ROCHA, BRUNO ALVES; MORAES DE OLIVEIRA, ANDERSON RODRIGO; BARBOSA, JR., FERNANDO. A fast and simple air-assisted liquid-liquid microextraction procedure for the simultaneous determination of bisphenols, parabens, benzophenones, triclosan, and triclocarban in human urine by liquid chromatography-tandem mass spectrometry. Talanta, v. 183, p. 94-101, JUN 1 2018. (13/23710-3)

MARTINS, JR., AIRTON CUNHA; GALLIMBERTI, MATHEUS; DE OLIVEIRA SOUZA, VANESSA CRISTINA; ROCHA, BRUNO ALVES; BARBOSA, JR., FERNANDO. Biomonitoring for uranium exposure among young children living in nineteen states across five regions of Brazil. JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, v. 317, n. 2, p. 779-785, AUG 2018. (13/23710-3)

ROCHA, BRUNO A.; ASIMAKOPOULOS, ALEXANDROS G.; BARBOSA, JR., FERNANDO; KANNAN, KURUNTHACHALAM. Urinary concentrations of 25 phthalate metabolites in Brazilian children and their association with oxidative DNA damage. Science of The Total Environment, v. 586, p. 152-162, MAY 15 2017. (15/20928-3, 13/23710-3)

SILVEIRA, ROMENA SANGLARD; ROCHA, BRUNO ALVES; RODRIGUES, JAIRO LISBOA; BARBOSA, JR., FERNANDO. Rapid, sensitive and simultaneous determination of 16 endocrine-disrupting chemicals (parabens, benzophenones, bisphenols, and triclocarban) in human urine based on microextraction by packed sorbent combined with liquid chromatography tandem mass spectrometry (MEPS-LC-MS/MS). Chemosphere, v. 240, FEB 2020. (14/50867-3, 18/24069-3, 14/19914-5, 16/02949-6, 13/23710-3)

BRUNO ALVES ROCHA; MARIANA ZUCCHERATO BOCATO; ELDER FRANCISCO LATORRACA; JOÃO PAULO BIANCHI XIMENEZ; FERNANDO BARBOSA JR.. A survey of parabens in commercial baby wipes from Brazil and estimation of daily exposure. Química Nova, v. 43, n. 4, p. 442-446, 2020-06-10. (13/23710-3, 14/19914-5, 14/50867-3, 16/02949-6)

ROCHA, BRUNO ALVES; AZEVEDO, LARA FERREIRA; GALLIMBERTI, MATHEUS; CAMPIGLIA, ANDRES DOBAL; BARBOSA, JR., FERNANDO. HIGH LEVELS OF BISPHENOL A AND BISPHENOL S IN BRAZILIAN THERMAL PAPER RECEIPTS AND ESTIMATION OF DAILY EXPOSURE. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 78, n. 18, p. 1181-1188, SEP 17 2015. (13/23710-3)

DE OLIVEIRA, MARIANA LEPRI; ROCHA, BRUNO ALVES; DE OLIVEIRA SOUZA, VANESSA CRISTINA; BARBOSA, JR., FERNANDO. Determination of 17 potential endocrine-disrupting chemicals in human saliva by dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry. Talanta, v. 196, p. 271-276, MAY 1 2019. (14/50867-3, 13/23710-3, 14/19914-5, 16/02949-6)