Although, Brazil lead in amphibian diversity, they are exposed to contaminants that may cause reduction in the number of species. Polycyclic Aromatic Hydrocarbons (PAHs) are contaminants generated from combustion and are present in the environment. Benzo[alfa]pyrene (BAP) is a mutagenic and carcinogenic compound metabolized in the liver and transported through it to the bloodstream. In this organ there is a cell type, the melanomacrófago, defense cells containing pigment and can store melanin, hemosiderin and lipofuscin, and some products derived from cell catabolism, and some products derived from cell catabolism. As blood cells of amphibians are: erythrocytes and leukocytes (basophils, eosinophils, monocytes and thrombocytes). There is a pattern of morphological and quantitative variability of these cells, however, exposure to chemical pollutants can induce changes in hematological parameters of animals. Thus, in two morphologically distinct species, the research objectives are to evaluate the effect of benzo[±]pyrene in melanomacrophages liver; quantify mast cells, making blood analysis (in Leptodactylus fuscus only) the effects of benzo[±]pyrene; do descriptive ultrastructural analysis of the liver. For this, 36 animals of Physalaemus cuvieri and 36 Leptodactylus fuscus species will be used. The experiment will be performed in the experimental time of 24h, 7 and 13 days, where animals receive the concentration of 2mg/kg BAP. The animals will be euthanized and liver will be weighed on an analytical balance. Shall follow-up the procedures included in historesin for analysis by light microscopy and also inclusion in Araldite for analysis in transmission electron microscopy.
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