Glutamatergic metabotropic receptors role in lateral hypothalamus and perifornicial area (LH/PFA) in daily variations control of ventilatory responses promoted by central chemoreflex activation

• Grant number: 14/17862-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2014
• End date: August 31, 2015
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Mirela Barros Dias
• Grantee: Laísa Taís Cabral Rodrigues Xavier
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 13/04216-8 - Neural mechanisms involved in the diurnal variation of the central chemoreflex control, AP.JP

Abstract

Evidence suggests the existence of daily variations in the control of the central chemoreflex, and has been attributed to orexinergic neurons of the lateral hypothalamus and perifornical area (LH / PFA) as a mediator of circadian variations in central chemorreflex, but the mechanisms involved remain to be elucidated. This project aims to investigate the possible role of metabotropic glutamate receptors in the LH/PFA in the modulation of the ventilatory response to hypercapnia in awake and sleeping rats during the dark and light period of the light/dark cycle. In unanaesthetized Wistar rats, ventilation will be measured using a whole-body plethysmograph. Animals will be submitted to microinjections of alpha-methyl-4-carboxyphenylglycine (MCPG; metabotropic glutamate antagonist) and its vehicle in the LH/PFA, and the effects of these approaches on ventilation will be evaluated in room air and 7% CO2 on sleep and wakefulness during both the dark and light periods. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CABRAL RODRIGUES, LAISA TAIS; DA SILVA, ELIANDRA NUNES; HORTA-JUNIOR, JOSE DE ANCHIETA C.; GARGAGLIONI, LUCIANE H.; DIAS, MIRELA B.. Glutamate metabotropic receptors in the lateral hypothalamus/perifornical area reduce the CO2 chemoreflex. Respiratory Physiology & Neurobiology, v. 260, p. 122-130, FEB 2019. (14/17862-8, 13/04216-8, 16/05174-5)