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Employment of early biomarkers in the evaluation of renal injury in rats subjected to ischemia / reperfusion under inhalation anesthesia with isoflurane.

Grant number: 14/10002-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2014
Effective date (End): August 31, 2015
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Norma Sueli Pinheiro Módolo
Grantee:Guilherme Augusto Pereira Pucci
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Background and Objective: Ischemia / reperfusion is a major cause of acute renal failurecommon in the perioperative period and in critically ill patients admitted to intensive careunits. The restoration of blood flow may aggravate the injury by ischemia and reperfusionmechanism (I / R). The aim of this study is to monitor renal function in rats anesthetized withisoflurane and subjected to an experimental model of I / R, in order to evaluate possible renalprotection from pre - infusion of fenoldopam and clonidine, by employing biomarkers ofinjury which are manifested earlier (NGAL, IL -18, KIM - 1 and L- FABP) and correlatethem with the RIFLE criteria determined by the measurement of serum creatinine.Methods: 40 Wistar rats will be selected and allocated into four random groups: IschemiaGroup (right nephrectomy, infusion of saline and maneuvers I / R , n = 10 ); FenoldopamGroup (right nephrectomy, infusion of fenoldopam, 5¼g.kg -1.min, intravenous infusion onehour before the ischemic and maneuvering procedure I / R, N = 10); Sham Group (laparotomyand infusion of saline , n = 10) and Clonidine Group (right nephrectomy, oral clonidine, 150ug / kg / day / 2 days and four hours before the procedure ischemic, n = 10). Animals will beanesthetized with isoflurane. During the experimental procedure, weight (W), heart rate (HR),systolic blood pressure (SBP), diastolic (DBP) and mean (MAP) , intraoperative temperature(T) will be evaluated in moments M0 (initial monitoring), M1 (initial time), M2 (afterreperfusion), M3 (2 hours after the start of reperfusion), M4 (4 hours after the start ofreperfusion), M5 (6 hours after the start of reperfusion), M6 (8 hours after the start ofreperfusion) and M7 (24 hours after M1). Plasma dosages of NGAL, IL -18, KIM - 1, LFABPand creatinine will be held in moments M1, M2, M3, M4, M5, M6 and M7. Animalswill be placed in metabolic cages for urine collection between M3 and M7 and dosages ofurinary NGAL, IL -18, KIM-1 and L- FABP. The kidneys will be histologically analyzed fordetermination of acute tubular necrosis