Assessment of carious lesions and dental biofilm by fluorescence in metabolic syndrome patients underwent to bariatric surgery: a prospective cohort study

• Grant number: 14/09550-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2014
• End date: May 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Silvia Helena de Carvalho Sales Peres
• Grantee: Joselene Martinelli Yamashita
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The obesity and metabolic syndrome have increased in worring proportions in worldwide. Besides the systemic consequences, obesity and its comorbidities have been associated with oral diseases and the aim of this study will be to identify oral problems in obese subjects with metabolic syndrome before and after bariatric surgery. The sample will be composed by two groups: experimental group (EG) and control group (CG). The EG will be composed by 125 morbidly obese patients with metabolic syndrome attended at Amaral Carvalho Hospital located in Jau - SP, and the CG will be composed by 125 normal individuals. The groups will be assessed in two periods (baseline and 6 months after bariatric surgery in EG and 6 months after the initial assessment in CG). The anthropometric assessment of individuals will be performed by BMI and waist circumference. The green fluorescence (QLF - Quantitative Light-induced Fluorescence), ICDAS II and DMTF will be used to assess dental caries. The dental plaque will be assessed through red fluorescence (QLF).Stimulated salivary flow will be evaluated by mL/mim. Index of Tongue Coating will be used to assess the presence of tongue coating and samples will be collected with an appropriate disposable tape and stored in eppendorf tubes at -80°C up to analysis of the presence of F. nucleatum, T. forsythia, P. gingivalis, P. intermedia e S mutans by q-PCR. In statistical analysis, first the normality test be applied and then the appropriate statistical test will be choose. In case of having a normal distribution, the parametric t-Student test will be used to compare the groups, otherwise the no-parametric Mann-Whitney test will be applied (P<0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YAMASHITA, JOSELENE MARTINELLI; DE MOURA-GREC, PATRCIA GARCIA; DE FREITAS, ADRIANA RODRIGUES; SALES-PERES, ARSENIO; GROPPO, FRANCISCO CARLOS; CENEVIVA, REGINALDO; DE CARVALHO SALES-PERES, SILVIA HELENA. Assessment of Oral Conditions and Quality of Life in Morbid Obese and Normal Weight Individuals: A Cross-Sectional Study. PLoS One, v. 10, n. 7, JUL 15 2015. (14/09550-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

YAMASHITA, Joselene Martinelli. Evaluation of caries lesions and dental biofilm by fluorescence, in patients with metabolic syndrome submitted to bariatric surgery: a prospective cohort study. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.