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Chemical and biological assessment of composting produced at the Fundação Parque Zoológico de São Paulo (FPZSP).

Grant number: 14/07037-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): October 01, 2014
Effective date (End): September 30, 2018
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal researcher:Luiz Juliano Neto
Grantee:Luciana Teresa Dias Cappelini
Home Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated scholarship(s):17/08038-8 - Search of potential non-ribosomal peptide producers (NRPS) in the compost from the foundation Parque Zoológico de São Paulo using iChip and Prism, BE.EP.PD


Alternatives for the disposing of organic waste generated by our society are always a necessity. Among this alternatives the Fundação Parque Zoológico de São Paulo (FPZSP) uses organic waste generated daily in the park for production of composting, which is subsequently used inside of the ZOO and also its farm (Unidade de Produção Rural -UPR) where it is produced most of the food consumed by the animals. Composting is the result of digestion of organic matter by the action of macro and mesofauna and of different communities of microorganisms, and this result supplies the plants with absorbable forms of nutrients that they need. Besides composting, there is also the generation of leachate that is generated from the cooling water of composters, still little exploited in conditions FPZSP. The aim of this work is to evaluate the peptidases and enzymes involved in the process of fixation, nitrification, denitrification and ammonification of nitrogen. We will use fluorescence resonance energy transfer (FRET) peptide libraries and will develop a methodology for direct extraction of enzymes from different samples of composting process # ZC4 that will be analyzed using a targeted proteomics LC - MS / MS. This procedure is intended to evaluate the performance of nitrifying organisms and also of peptidases in different stages of composting process. Another relevant aim of this proposal is the possibility to mine the proteins that we will identify in biochemical and proteomic analysis in the metagenomic data that will be obtained with the same samples that we will use in our work. This metagenomic analysis is part of project coordinated by Prof. Dr. João Carlos Setubal (Processo FAPESP - Tematico: 2011/508706) and we will have a unique chance to experiment a proteogenomic analysis exercise in a complex system as the composting process.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RAMOS, PATRICIA L.; KONDO, MARCIA Y.; SANTOS, SAARA M. B.; DE VASCONCELLOS, SUZAN P.; ROCHA, RAFAEL C. S.; DA CRUZ, JOAO B.; EUGENIO, PATRICIA F. M.; CABRAL, HAMILTON; JULIANO, MARIA A.; JULIANO, LUIZ; SETUBAL, JOAO C.; DA SILVA, ALINE M.; CAPPELINI, LUCIANA T. D. A Tropical Composting Operation Unit at SAo Paulo Zoo as a Source of Bacterial Proteolytic Enzymes. Applied Biochemistry and Biotechnology, v. 187, n. 1, p. 282-297, JAN 2019. Web of Science Citations: 2.
DIAS CAPPELINI, LUCIANA TERESA; MENEGOCI EUGENIO, PATRICIA DE FATIMA; GOMES CARNEIRO LEAO, PAULO AUGUSTO; ALBERICE, JULIANA VIEIRA; URBACZEK, ANA CAROLINA; ASSUNCAO, NILSON ANTONIO; JULIANO, LUIZ; CARRILHO, EMANUEL. Capillary electrophoresis coupled to contactless conductivity detection for analysis of amino acids of agricultural interest in composting. ELECTROPHORESIS, v. 37, n. 19, SI, p. 2449-2457, OCT 2016. Web of Science Citations: 4.

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