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Chemical identification and evaluation of antitumoral potential of metabolites of Guarea macrophylla ssp. tuberculata (Meliaceae)

Grant number: 14/08961-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): September 01, 2014
Effective date (End): August 31, 2016
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:João Henrique Ghilardi Lago
Grantee:Geanne Alexsandra Alves Conserva
Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil


Plants displayed a fundamental importance in the lives of all people. Natural products extracted from plants, have emerged as important sources of new therapeutic agents used in the treatment of several diseases, including cancer. Therefore, several studies were conducted to analysis of active principles of plants, with selective tumor cell activity, as well as investigating the mechanism of action of drugs with pharmacological action. Thus, this project aims to identify, isolate and elucidate the structures of compounds which showed cytotoxic activity from ethanol extract of leaves of Guarea macrophylla ssp. tuberculata (Meliaceae) against several cell lines (melanoma, breast adenocarcinoma, leukemia and human carcinoma). To conduct this study, the bioassay-guided phytochemical fractionation will be employed to the isolation and structural characterization of the active compounds present in the extracts. Furthermore, we intend to elucidate the mechanism of action related to tumor cell apoptosis through tests performed in vitro by evaluating the degradation of DNA, tunel assay, analysis of apoptotic morphological changes by transmission electron microscopy - (TEM), determination of mitochondrial membrane potential (Èm) and determination of intracellular superoxide anion. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CONSERVA, GEANNE ALEXANDRA A.; GIROLA, NATALIA; FIGUEIREDO, CARLOS R.; AZEVEDO, RICARDO A.; MOUSDELL, SASHA; SARTORELLI, PATRICIA; SOARES, MARISI G.; ANTAR, GUILHERME M.; LAGO, JOAO HENRIQUE G.. Terpenoids from Leaves of Guarea macrophylla Display In Vitro Cytotoxic Activity and Induce Apoptosis In Melanoma Cells. Planta Medica, v. 83, n. 16, p. 1289-1296, . (14/08961-2)
UENO, ANDERSON K.; BARCELLOS, ALINE F.; COSTA-SILVA, THAIS A.; MESQUITA, JULIANA T.; FERREIRA, DAIANE D.; TEMPONE, ANDRE G.; ROMOFF, PAULETE; ANTAR, GUILHERME M.; LAGO, JOAO HENRIQUE G.. Antitrypanosomal activity and evaluation of the mechanism of action of diterpenes from aerial parts of Baccharis retusa (Asteraceae). Fitoterapia, v. 125, p. 55-58, . (15/23403-9, 15/11936-2, 14/08961-2)

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