Study of inflammatory and angiogenic processes in heterologous implants of rats using porcine acellular matrices

• Grant number: 14/18557-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2014
• Effective date (End): June 30, 2016
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Sonia Maria Oliani
• Grantee: Andréia Rodrigues de Moraes
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated scholarship(s): 15/03916-1 - Production and characterization of acellular matrices of porcine skin using a time and cost effective method, BE.EP.IC

Abstract

Tissue Engineering is an important field of modern medicine that develops biological substitutes for tissues and organs. The advances in this area have led to the development of various products, both synthetic and biological, with the goal of improving the quality of life of the patient, either through restoration and maintenance of tissues or improving the functions of tissues and transplanted organs. Designated as skin substitutes, equivalents of the epidermis and dermis have been investigated and used clinically for several years. However, acellular biological materials immunologically compatible and suited to the repair of skin, both as fill (dermis) or support and protection (epidermis), need to be carefully implemented. The implementation of these biomaterials to the host organism provokes responses that depend on the material used. The success of implants depends on inflammatory and angiogenic processes. Once started these considerations, using matrices from porcine skin dermis, completely decellularized by economically viable techniques of removal nucleic materials, our goal is to evaluate the biocompatibility of the scaffolds after subcutaneous implantation in rats. The following aspects will be evaluated by immunohistochemical techniques: inflammation (neutrophils, macrophages M1 / M2 and expression of annexin A1 anti-inflammatory protein - ANXA1), angiogenesis (neovascularization), and apoptosis in heterografts. These data may indicate important aspects in understanding the regenerative and healing processes of heterografts and possibly new therapeutic alternatives.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIMURA, KALLYNE K. O.; MORAES, ANDREIA R.; MIRANDA, ALINE C.; GRECO, REBECCA; ANSARI, TAHERA; SIBBONS, PAUL; GRECO, KARIN V.; OLIANI, SONIA M.. Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling. SCIENTIFIC REPORTS, v. 6, OCT 11 2016. (12/13041-4, 12/21603-2, 14/18557-4)