Dietary effects on the sperm epigenome

• Grant number: 14/21097-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: December 01, 2014
• End date: September 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Flávio Vieira Meirelles
• Grantee: Juliano Rodrigues Sangalli
• Supervisor: Oliver Joaquin Rando
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Institution abroad: University of Massachusetts Medical School (UMMS), United States
• Associated to the scholarship: 13/06673-7 - Use of histones deacetylases inhibitors in bovine somatic cell nuclear transfer., BP.DR

Abstract

It is increasingly apparent that ancestral environmental conditions can affect phenotypes of future generations. In particular, a great deal of evidence indicates that parental dietary conditions have significant effects on metabolic phenotypes in children. In humans, epidemiological studies show that ancestral nutritional status can affect the risk of cardiovascular diseases of offspring through the male germline. In rodents, Rando's lab and others have shown that paternal diet can affect metabolic phenotypes in offspring. As males contribute little beyond sperm to their offspring in these paradigms, it is of great interest to understand the mechanisms by which paternal environment can affect phenotype in progeny.Mammalian sperm carry several known or hypothesized carriers of epigenetic information, including methylated cytosines, histones, and RNA molecules. Here, we propose a multi-pronged, interconnected series of experiments to comprehensively map the sperm epigenome, to identify environmental effects on the sperm epigenome, and to identify epigenomic changes in sperm that are maintained during fertilization and early embryogenesis. Our efforts will provide the foundation for understanding mammalian epigenetic inheritance in general, and will identify potential mechanisms by which metabolic disease states are transmitted to offspring. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANGALLI, JULIANO RODRIGUES; SAMPAIO, RAFAEL VILAR; DEL COLLADO, MAITE; DA SILVEIRA, JULIANO COELHO; CAMARA DE BEM, TIAGO HENRIQUE; PERECIN, FELIPE; SMITH, LAWRENCE CHARLES; MEIRELLES, FLAVIO VIEIRA. Metabolic gene expression and epigenetic effects of the ketone body beta-hydroxybutyrate on H3K9ac in bovine cells, oocytes and embryos (vol 8, 13766, 2018). SCIENTIFIC REPORTS, v. 8, p. 1-pg., 2018-11-16. (16/13416-9, 14/22887-0, 14/21034-3, 12/50533-2, 14/50947-7, 13/06673-7, 13/08135-2, 14/21097-5)

SANGALLI, JULIANO RODRIGUES; SAMPAIO, RAFAEL VILAR; DEL COLLADO, MAITE; DA SILVEIRA, JULIANO COELHO; CAMARA DE BEM, TIAGO HENRIQUE; PERECIN, FELIPE; SMITH, LAWRENCE CHARLES; MEIRELLES, FLAVIO VIEIRA. Metabolic gene expression and epigenetic effects of the ketone body beta-hydroxybutyrate. SCIENTIFIC REPORTS, v. 8, SEP 13 2018. (13/08135-2, 16/13416-9, 13/06673-7, 12/50533-2, 14/21097-5, 14/22887-0, 14/21034-3)