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Simultaneous determination of biomarkers of renal function in urine using microfluidic paper: based analytical device

Grant number: 14/17749-7
Support type:Scholarships in Brazil - Master
Effective date (Start): November 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Helena Redigolo Pezza
Grantee:Eduardo Luiz Rossini
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Nitrogen is mainly present in the human body as a constituent of proteins and nucleic acids. The catabolism of these substances form nitrogenous compounds known as non-protein. Among the various non-protein nitrogenous compounds known, uric acid and creatinine may be used as biomarkers of renal function in humans. Creatinine is derived from the nonenzymatic reaction of dehydration of creatine or phosphocreatine dephosphorylation during the process of muscle contraction; its concentration is used as a clinical marker to assess renal function. Uric acid is formed from the metabolism of purine bases, as a low soluble compound, deposition in the end joints or other soft tissues in a case generates known as gout. Thus, creatinine and uric acid are constantly measured in laboratory tests of urine. The methodologies used for the determination of these two compounds ranging from classical methods to the use of HPLC, electrophoresis and flow analysis, namely, little portable techniques and some high cost. Thus, the possibility of the use of microfluidic paper - based analytical devices (¼PAD) for the determination of creatinine and uric acid will be studied, it is a cheap, clean, fast, portable and can be used in remote locations to function evaluation method kidney. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROSSINI, EDUARDO LUIZ; MILANI, MARIA IZABEL; CARRILHO, EMANUEL; PEZZA, LEONARDO; PEZZA, HELENA REDIGOLO. Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device. Analytica Chimica Acta, v. 997, p. 16-23, JAN 2 2018. Web of Science Citations: 17.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ROSSINI, Eduardo Luiz. Determinação simultânea de biomarcadores de função renal em urina utilizando dispositivos analítico microfluídico em papel. 2016. 125 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Química..

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