Characterization of plasmid-mediated quinolone resistance genes in Escherichia coli isolated from poultry and swine production chain

• Grant number: 14/11523-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2014
• End date: April 30, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Terezinha Knöbl
• Grantee: Marcos Paulo Vieira Cunha
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The selective pressure caused by the antimicrobials used as growth promoters in animal production is a current concern worldwide. Recently, several studies have shown that strains of pathogenic and/or multidrug-resistant bacteria are selected in the context of animal production. Among the antibiotics that have showing increased levels of resistance are the quinolones and fluoroquinolones. Until 1998 the resistance to quinolones was assigned to only mechanisms mutation in DNA gyrase and topoisomerase IV in bacterial DNA. The emergence of resistance to quinolones mediated by plasmids and dissemination of these elements brought the issue of animals serve as a source of genes for resistance to this class. Several studies show that the consumption of animal foods, especially poultry and pork, and proximity to these animals as a fundamental element in the epidemiological chain of human infections by bacteria such Salmonella spp, Campylobacter spp., and more recently by Escherichia coli. Taking into account the importance of the issue for public health, the importance of poultry and pigs in the Brazilian economy and the dearth of research on this topic in Brazil, this project is to evaluate the molecular epidemiology and phenotypically and genotypically characterize the resistance plasmids quinolones present in Escherichia coli isolated from different stages of swine and poultry production in Brazil. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, MIRIAM R.; SELLERA, FABIO P.; CUNHA, MARCOS P. V.; LOPES, RALF; CERDEIRA, LOUISE; LINCOPAN, NILTON. Emergence of CTX-M-27-producing Escherichia coli of ST131 and clade C1-M27 in an impacted ecosystem with international maritime traffic in South America. Journal of Antimicrobial Chemotherapy, v. 75, n. 6, p. 1647-1649, JUN 2020. (15/13527-2, 16/08593-9, 14/11523-7)

DE OLIVEIRA, MIRELA C. V.; CAMARGO, BEATRIZ Q.; CUNHA, MARCOS P. V.; SAIDENBERG, ANDRE BECKER; TEIXEIRA, RODRIGO H. F.; MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; GOMES, VASCO T. M.; CHRIST, ANA P. G.; BARBOSA, MIKAELA R. F.; et al. Free-Ranging Synanthropic Birds (Ardea alba and Columba livia domestica) as Carriers of Salmonella spp. and Diarrheagenic Escherichia coli in the Vicinity of an Urban Zoo. VECTOR-BORNE AND ZOONOTIC DISEASES, v. 18, n. 1, p. 65-69, JAN 2018. (14/11523-7, 15/26159-1, 16/12641-9)

DAVIES, Y. M.; CUNHA, M. P. V.; OLIVEIRA, M. G. X.; OLIVEIRA, M. C. V.; PHILADELPHO, N.; ROMERO, D. C.; MILANELO, L.; GUIMARAES, M. B.; FERREIRA, A. J. P.; MORENO, A. M.; et al. Virulence and antimicrobial resistance of Klebsiella pneumoniae isolated from passerine and psittacine birds. AVIAN PATHOLOGY, v. 45, n. 2, p. 194-201, 2016. (14/11523-7)

VIEIRA CUNHA, MARCOS PAULO; SAIDENBERG, ANDRE BECKER; MORENO, ANDREA MICKE; PIANTINO FERREIRA, ANTONIO JOSE; MIDOLLI VIEIRA, MONICA APARECIDA; TARDELLI GOMES, TANIA APARECIDA; KNOBL, TEREZINHA. Pandemic extra-intestinal pathogenic Escherichia coli (ExPEC) clonal group O6-B2-ST73 as a cause of avian colibacillosis in Brazil. PLoS One, v. 12, n. 6, JUN 8 2017. (14/11523-7)

CUNHA, MARCOS P. V.; LINCOPAN, NILTON; CERDEIRA, LOUISE; ESPOSITO, FERNANDA; DROPA, MILENA; FRANCO, LETICIA S.; MORENO, ANDREA M.; KNOEBL, TEREZINHA. Coexistence of CTX-M-2, CTX-M-55, CMY-2, FosA3, and QnrB19 in Extraintestinal Pathogenic Escherichia coli from Poultry in Brazil. Antimicrobial Agents and Chemotherapy, v. 61, n. 4, APR 2017. (14/11523-7, 16/12641-9, 15/21325-0)

SPINDOLA, MARIA G.; CUNHA, MARCOS P. V.; MORENO, LUISA Z.; AMIGO, CRISTINA R.; SILVA, ANA P. S.; PARRA, BEATRIZ M.; POOR, ANDRE P.; DE OLIVEIRA, CAROLINA H.; PEREZ, BARBARA P.; KNOBL, TEREZINHA; et al. Genetic diversity, virulence genotype and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from sows. VETERINARY QUARTERLY, v. 38, n. 1, p. 79-87, OCT 26 2018. (13/17136-2, 13/16946-0, 14/11523-7, 16/25745-7, 17/09515-4, 11/22608-5)

SAIDENBERG, ANDRE BECKER S.; VAN VLIET, ARNOUD H. M.; STEGGER, MARC; JOHANNESEN, THOR BECH; SEMMLER, TORSTEN; CUNHA, MARCOS; DE O. SILVEIRA, ALESSANDRO C.; ANZAI, ELEINE KUROKI; SCALETSKY, ISABEL C. A.; DALSGAARD, ANDERS; et al. Genomic analysis of the zoonotic ST73 lineage containing avian and human extraintestinal pathogenic Escherichia coli (ExPEC). Veterinary Microbiology, v. 267, p. 8-pg., 2022-04-01. (14/11523-7)

CUNHA, MARCOS P. V.; DAVIES, YAME M.; CERDEIRA, LOUISE; DROPA, MILENA; LINCOPAN, NILTON; KNOBL, TEREZINHA. Complete DNA Sequence of an IncM1 Plasmid Bearing the Novel qnrE1 Plasmid-Mediated Quinolone Resistance Variant and bla(CTX-M-8) from Klebsiella pneumoniae Sequence Type 147. Antimicrobial Agents and Chemotherapy, v. 61, n. 9, SEP 2017. (16/12641-9, 15/21325-0, 14/11523-7)

SAIDENBERG, ANDRE BECKER S.; STEGGER, MARC; PRICE, LANCE BRADLEY; JOHANNESEN, THOR BECH; AZIZ, MALIHA; CUNHA, MARCOS P. V.; MORENO, ANDREA M.; KNOBL, TEREZINHA. mcr-Positive Escherichia coli ST131-H22 from Poultry in Brazil. Emerging Infectious Diseases, v. 26, n. 8, p. 1951-1954, AUG 2020. (14/11523-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CUNHA, Marcos Paulo Vieira. Emerging antimicrobial resistance determinants in poultry and swineEscherichia coli isolates from clinical, fecal and meat sources.. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.