Analysis of kinship degree and genetic variability of pacu (Piaractus mesopotamicus) through SNPs markers: tools for genetic improvement

• Grant number: 14/12412-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2014
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Diogo Teruo Hashimoto
• Grantee: Vito Antonio Mastrochirico Filho
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

With the increasing number of overfished species, as well as drastic changes in their natural environments, the development of a correct exploitation of aquaculture resources could support the demand of animal protein. Breeding programs can ensure maximization of productive performance in farming systems, through optimizing the yield of the target species. However, initial studies of genetic variability using molecular markers is essential, as a resource to begin a breeding program with a wide variety of genotypes, as well as to avoid the genetic bottleneck and inbreeding depression during the generations. For this purpose, SNPs (single nucleotide polymorphisms) are considered the most suitable molecular markers nowadays, since they are the most abundant polymorphism in any organism. Pacu (Piaractus mesopotamicus) is considered one of the fish species with higher production and commercial value to the national aquaculture; consequently, it is a target species for breeding programs. This study aims to analyse the pairwise kinship degree and genetic variability of pacu stocks using SNPs markers obtained by next-generation sequencing. The characterization of the liver transcriptome will be performed in about 20 individuals from different fish farm in Brazil and natural environment. For the SNPs validation, the analysis will be done in approximately 30 wild individuals. Finally, with the establishment of SNPs, we intend to analyse farming broodstock of pacu in Brazilian fish farms (approximately 200 samples), in order to know the genetic profile of these animals and improve the genetic management of production systems. Genetic studies focusing on better understanding of the pacu genomes in farming stocks are considered essential for aquaculture, especially to provide resources for the development of management and breeding programs of this species. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HASHIMOTO, D. T.; HATA, M. E.; SATO, L. S.; JORGE, P. H.; MASTROCHIRICO-FILHO, V. A.; MARTINEZ, P.; FORESTI, F.; PORTO-FORESTI, F.. TRANSCRIPTOME CHARACTERIZATION AND SNP DISCOVERY IN SPECIES OF SERRASALMIDAE: GENETIC SUBSIDIES FOR THE BRAZILIAN AQUACULTURE. Aquaculture, v. 472, p. 1-pg., 2017-04-01. (14/05732-2, 14/12412-4, 14/03772-7)

ANTONIO MASTROCHIRICO-FILHO, VITO; DEL PAZO, FELIPE; ELISSA HATA, MILENE; VANINA VILLANOVA, GABRIELA; FORESTI, FAUSTO; VERA, MANUEL; MARTINEZ, PAULINO; PORTO-FORESTI, FABIO; TERUO HASHIMOTO, DIOGO. Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs. GENES, v. 10, n. 9, SEP 2019. (14/03772-7, 14/12412-4)

MASTROCHIRICO-FILHO, VITO ANTONIO; HATA, MILENE ELISSA; SATO, LUCAS SEITI; JORGE, PAULO HENRIQUE; FORESTI, FAUSTO; RODRIGUEZ, MANUEL VERA; MARTINEZ, PAULINO; PORTO-FORESTI, FABIO; HASHIMOTO, DIOGO TERUO. SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus. CONSERVATION GENETICS RESOURCES, v. 8, n. 2, p. 109-114, JUN 2016. (14/03772-7, 14/12412-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MASTROCHIRICO FILHO, Vito Antonio. Genetic variability and parentage assignment assessed by SNPs in stocks of the fish pacu (Piaractus mesopotamicus). 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.