Investigation of Pseudomonas aeruginosa respiratory infection in patients with cystic fibrosis in a reference center in Brazil: evaluation of the utility of four different diagnostic methods

• Grant number: 14/00007-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): December 01, 2014
• Effective date (End): August 31, 2018
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Carlos Emilio Levy
• Grantee: Renan Marrichi Mauch
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 15/26043-3 - Avidity of antibodies against Pseudomonas aeruginosa during respiratory infection and the humoral immune response to different pathogens during pulmonary exacerbation in cystic fibrosis, BE.EP.DR

Abstract

Cystic fibrosis (CF) is a genetic disease resulting from dysfunction of the CFTR protein, essential for the transport of ions and water across the cell membrane, and the lung disease is the most prominent clinical manifestation. Dehydration of the respiratory secretions occurs resulting in increased viscosity, impairing of mucociliary transport, developing chronic low airways obstruction, with bacterial colonization followed by infections, particularly with Pseudomonas aeruginosa, the most relevant bacterium for CF patients. The gold standard method for detection of P. aeruginosa is the culture of sputum or oropharyngeal swab, which have limitations, like non representative material and low detection power, leading to the need of methods with higher accuracy. The specific serological techniques and real-time PCR have shown high positive and negative predictive values and greater capacity for early detection that may be useful for this purpose. Besides, the detection of secretory IgA (sIgA) in saliva has recently emerged as a method with potential ability for the diagnosis of P. aeruginosa infection in the upper airways, which may precede pulmonary infection. In this context, the aim of the present study is to evaluate the usefulness of three laboratorial techniques - real-time PCR of respiratory samples, ELISA (enzyme-linked immunosorbent assay) for detection of anti-P. aeruginosa antibodies (serum IgG and sIgA) - in relation to the microbiological respiratory culture for the diagnosis of P. aeruginosa respiratory infection in patients recently diagnosed with CF in the Campinas State University Hospital reference center (HC-Unicamp). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAUCH, RENAN MARRICHI; ROSSI, CLAUDIO LUCIO; NOLASCO DA SILVA, MARCOS TADEU; AIELLO, TALITA BIANCHI; RIBEIRO, JOSE DIRCEU; RIBEIRO, ANTONIO FERNANDO; HOIBY, NIELS; LEVY, CARLOS EMILIO. Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients. MEDICAL MICROBIOLOGY AND IMMUNOLOGY, v. 208, n. 2, p. 205-213, APR 2019. (14/00007-8)

MAUCH, RENAN M.; ROSSI, CLAUDIO L.; AIELLO, TALITA B.; RIBEIRO, JOSE D.; RIBEIRO, ANTONIO F.; HOIBY, NIELS; LEVY, CARLOS E.. Secretory IgA response against Pseudomonas aeruginosa in the upper airways and the link with chronic lung infection in cystic fibrosis. PATHOGENS AND DISEASE, v. 75, n. 6, AUG 2017. (14/00007-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MAUCH, Renan Marrichi. Pseudomonas aeruginosa infection in the cystic fibrosis airways: early detection and functionality of the specific humoral immune response. 2018. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.