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Characterization of opioid receptors in the central nervous system and evaluation of analgesic and sedative effects of opioids in green iguanas (Iguana iguana)

Grant number: 14/09529-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2014
Effective date (End): July 31, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Adriano Bonfim Carregaro
Grantee:Thais Feres Bressan
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The aims of this study are to characterize and to localize opioid receptors (OR) in the Central Nervous System (CNS) of green iguanas (Iguana iguana), and to quantify sedative and analgesic effects of morphine and butorphanol at different doses in those animals. Characterization of the OR in the CNS will be perform by cloning and amplification of the OR cDNA, using 3` e 5` RACE PCR, to create a nucleotide sequence referent to MOR and KOR. OR cDNA will be used to detect and to localize those receptors in the CNS. This part will be performed by immunohistochemistry technique. To the second and third phase, 10 young animals, male or female, weighing between 50 and 150 grams, will be submitted to five treatments: morphine 10mg/kg (GM10), morphine 20mg/kg (GM20), butorphanol 10mg/kg (GB10), butorphanol 20mg/kg (GB20) and 0.5 mL of NaCl 0.9% (GC), by intramuscular route. Spontaneous locomotor activity will be performed before treatments (basal), 30 minutes, 1, 2, 3, 4, 6, 12 e 24 hours after treatments, by an open field technique. Analgesia will be performed at the same times described before, on another time, by noxious thermal stimulation (45 - 47°C). It will be expected to get the characterization and the localization of the MOR and KOR in the CNS of these animals, as well as to determine sedative and analgesic effects of morphine and butorphanol. Besides, it will be expected to determine the best morphine and butorphanol doses for this specie. (AU)