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Characterization of immunosensors array for detecting pathogenic micro-organisms in food by using a microfluidic system

Grant number: 14/21115-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2014
Effective date (End): November 30, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Ronaldo Censi Faria
Grantee:Diego Henrique Martucci
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The purpose of this project is the development of immunosensors array of low cost, sandwich, for multiplexed detection of disease-causing bacteria in food. Pathogenic bacteria are the major causative agents of foodborne disease (FBD). The sensor device is developed using primary antibodies immobilized on a film layer-by-layer (LBL) formed by gold nanoparticles (AuNPs) and políons in an arrangement of graphite electrodes. Magnetic particles modified massively HRP as electrochemical marker, and secondary antibodies are used for capture and pre-concentration of bacteria. For a method of analysis multiplexed use of voltammetry and chronoamperometry techniques will be-evaluated. For characterization of the films and the immobilization of antibodies process techniques specular reflectance spectroscopy in the infrared and atomic force microscopy (AFM) will be used. The microfluidic system designed to be low cost materials and evaluate it using samples spiked with synthetic DTA. The device will then be featured in FBD detect low detection limits, with high selectivity and low cost, allowing them to develop expertise in modern techniques of electrochemistry and spectroscopy.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, TASSIA REGINA; MARTUCCI, DIEGO HENRIQUE; FARIA, RONALDO CENSI. Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay. SENSORS AND ACTUATORS B-CHEMICAL, v. 255, n. 1, p. 684-691, FEB 2018. Web of Science Citations: 13.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.