Impact of protein restriction during pregnancy and lactation on rat prostate: relation between the signaling pathway of Insulin/IGF, development and aging

• Grant number: 14/08531-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2014
• End date: March 01, 2018
• Field of knowledge: Biological Sciences - Morphology - Histology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Luis Antonio Justulin Junior
• Grantee: Sérgio Alexandre Alcantara dos Santos
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 16/25033-7 - Organ culture as a model to study the dynamic of male genital system development: Wolffian duct and Prostate, BE.EP.DR

Abstract

Adverse pregnancy conditions may cause irreversible morphological and functional changes in the fetus, a phenomenon known as Gestational Fetal Programming (GFP). In utero protein restriction, a GFP model widely studied is responsible for low birth weight and the development of insulin resistance and type II diabetes in adulthood. The GFP also alters the levels of steroid hormones and growth factors, such as insulin/IGF f axis in the offspring. The Insulin/IGF axis has been related to the prostate development and glandular disorders during aging. This project aims to characterize the insulin/IGF signaling pathway in the prostate during normal development and aging, as well as possible changes in this pathway caused gestational protein restriction in these periods. Sprague Dawley rats (CTR group) born to mothers fed a normal diet (17 % protein) or low protein diet (6 % protein) during pregnancy (RPG group) or during pregnancy and lactation (RPGL group) are employed in this study. The ventral prostatic lobes (VP) and blood samples will be collected on postnatal day (PND) 21 and 540. Hormonal and morphological and morphometric parameters will be made. It will be applied immunocytochemical, western blotting and RT-PCR protocols to describe the insulin/IGF signaling pathway in all experimental groups. In the animals of 540 days, the data of insulin/IGF axis will be correlated with quantification of potential prostatic disorders observed in this age. This project is part of the ongoing regular project approved by FAPESP (Proc. no. 2013/24230-5). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALCANTARA SANTOS, SERGIO ALEXANDRE; LIMA CAMARGO, ANA CAROLINA; CONSTANTINO, FLAVIA BESSI; COLOMBELLI, KETLIN THASSIANI; FREDIANI PORTELA, LUIZ MARCOS; FIORETTO, MATHEUS NAIA; SOUZA VIEIRA, JOSE CAVALCANTE; PADILHA, PEDRO MAGALHAES; DE OLIVEIRA, MATEUS BETTA; FELISBINO, SERGIO LUIS; et al. Identification of potential molecular pathways involved in prostate carcinogenesis in offspring exposed to maternal malnutrition. AGING-US, v. 12, n. 20, p. 19954-19978, OCT 31 2020. (17/08715-0, 17/01063-7, 14/08531-8)

SANTOS, SERGIO A. A.; CAMARGO, ANA C.; CONSTANTINO, FLAVIA B.; COLOMBELLI, KETLIN T.; MANI, FERNANDA; RINALDI, JAQUELINE C.; FRANCO, SUELEN; PORTELA, LUIZ M. F.; DURAN, BRUNO O. S.; SCARANO, WELLERSON R.; et al. Maternal Low-Protein Diet Impairs Prostate Growth in Young Rat Offspring and Induces Prostate Carcinogenesis With Aging. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, v. 74, n. 6, p. 751-759, JUN 2019. (14/08531-8, 17/01063-7, 13/24230-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Sérgio Alexandre Alcantara dos. Impact of gestational and lactational protein restriction on rat prostate: Relation between the Insulin / IGF signaling pathway, development and aging.. 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.