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Influence of orthodontic tooth movement on periodontal disease progression in normal systemic health or in obesity

Grant number: 14/20715-7
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2015
Effective date (End): May 14, 2018
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Joni Augusto Cirelli
Grantee:Andressa Vilas Boas Nogueira
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):17/01358-7 - Influence of orthodontic tooth movement on periodontal disease progression in normal systemic health or in obesity, BE.EP.PD

Abstract

Many studies have been conducted to better understand the effects of periodontal disease and orthodontic tooth movement on periodontal tissues. However, the scientific literature shows no studies that assess these effects in periodontal tissues during obesity. The aim of this research project is to evaluate the effect of biomechanical force in the progression of periodontal disease in normal systemic health and in obesity conditions. In the in vitro study, human periodontal ligament cells (hPDL) will be exposed to Porphyromonas gingivalis ATCC 33277, NAMPT, adiponectin, cyclic tensional strain (CTS), and their combinations for 1 and 3 days. After the period of treatment, cells and culture medium will be collected for analysis of the inflammatory mediators COX2, IL-6, TNF-±, CCL2, MMP-1, and CCR2 by qPCR and ELISA. In the in vivo study, a total of 50 rats will be distributed in 5 experimental groups: control (C), experimental periodontal disease (P), obesity induction followed by P (OP), P induction followed by orthodontic movement (OMP), and O induction followed by P and OM (OMOP). The animals will be sacrificed after 7 days of OM begin. Alveolar bone loss will be measured by analysis of micro-computed tomography analysis. The periodontal ligament cells will be captured by laser capture microdissection (LCM) to evaluate the gene expression of the inflammatory markers: IL-1², IL-6, TNF-±, CCL2, CCR2, Itgam, visfatin, leptin, and adiponectin. Furthermore, the protein synthesis of these same molecules will be evaluated by immunohistochemistry.