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Investigation of the bioremediation potential to detoxify sludge from wastewater treatment plant, regarding estrogenic and dioxin-like compounds

Grant number: 14/14123-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2015
Effective date (End): October 17, 2018
Field of knowledge:Interdisciplinary Subjects
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Clovis Augusto Ribeiro
Grantee:Dânia Elisa Christofoletti Mazzeo Morales
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):17/10198-3 - Bioanalytical tools to investigate endocrine disrupting chemicals in sewage sludge, BE.EP.PD

Abstract

Sewage sludge (SS) is a residue derived from Wastewater Treatment Plants (WWTPs) that has a high pollution potential of soil. However, due to the high content of organic matter and nutrients, the SS is an excellent material for using as an agricultural fertilizer. However, the presence of toxic substances, such as estrogenic and dioxin-like ones, can cause serious impacts to the environment and associated organisms, even when present in low concentrations. From this context, the aim of this project is to seek bioassays for performing an efficient diagnosis of the biological effects of SS contaminants, associating its toxicity with substances present in this waste; develop low cost technologies for the degradation of emerging contaminants allowing the use of SS as agricultural additive; and characterize the microbial population involved in biodegradation. For this, samples of SS from WWTPs of two municipalities that use different operating systems will be submitted to the biostimulation process (for 2, 4 and 6 months) by the addition of agroindustrial (sugar cane bagasse) and domestic (coffee grounds) wastes. The identification of the biodegrader microorganisms will be made by Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) and the biological activity of endocrine disruptors and dioxin-like compounds will be evaluated by tests with recombinant yeast (estrogen and Ah receptor) and cultured human cells (E-screen and EROD). The chemical identification of target contaminants will be made by GC-MS/MS and LC-MS/MS. (AU)